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Involvement of the fusome-related protein Add1 in spermatogenesis of the Chinese mitten crab (Eriocheir sinensis) by organization of cytoskeleton

Involvement of the fusome-related protein Add1 in spermatogenesis of the Chinese mitten crab... The sperm nucleus of the decapod crustacean Chinese mitten crab, Eriocheir sinensis, is loose and fibrous, which is a non-condensed nucleus. Our purpose was to analyze the structural distribution of the fusome-related protein Add1 during the spermatogenesis in E. sinensis to better understand the non-condensed nucleus of E. sinensis. RT-qPCR and western blot results showed that Add1 was expressed at the transcriptional and translational level during spermatogenesis of E. sinensis. Immunofluorescence results showed that Add1 was widely distributed in the process of spermatogenesis, including spermatogonia, spermatocytes, spermatids, and sperm. In the initial stage of sperm nucleus decondensation of E. sinensis, Add1 was widely expressed in the cytoplasm of spermatogonia. In the process from stage III spermatids to sperm, Add1 is mainly expressed in the nucleus. With the continuous differentiation of cells, Add1 gradually transferred from the cytoplasm to the nucleus. Our results indicated that the fusome-related protein Add1 might be involved in the spermiogenesis through the construction of a new nuclear skeleton to participate in the decondensation of sperm nuclei and the maintenance and protection of non-condensed chromatin. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Aquaculture International Springer Journals

Involvement of the fusome-related protein Add1 in spermatogenesis of the Chinese mitten crab (Eriocheir sinensis) by organization of cytoskeleton

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References (34)

Publisher
Springer Journals
Copyright
Copyright © The Author(s), under exclusive licence to Springer Nature Switzerland AG 2022
ISSN
0967-6120
eISSN
1573-143X
DOI
10.1007/s10499-021-00825-z
Publisher site
See Article on Publisher Site

Abstract

The sperm nucleus of the decapod crustacean Chinese mitten crab, Eriocheir sinensis, is loose and fibrous, which is a non-condensed nucleus. Our purpose was to analyze the structural distribution of the fusome-related protein Add1 during the spermatogenesis in E. sinensis to better understand the non-condensed nucleus of E. sinensis. RT-qPCR and western blot results showed that Add1 was expressed at the transcriptional and translational level during spermatogenesis of E. sinensis. Immunofluorescence results showed that Add1 was widely distributed in the process of spermatogenesis, including spermatogonia, spermatocytes, spermatids, and sperm. In the initial stage of sperm nucleus decondensation of E. sinensis, Add1 was widely expressed in the cytoplasm of spermatogonia. In the process from stage III spermatids to sperm, Add1 is mainly expressed in the nucleus. With the continuous differentiation of cells, Add1 gradually transferred from the cytoplasm to the nucleus. Our results indicated that the fusome-related protein Add1 might be involved in the spermiogenesis through the construction of a new nuclear skeleton to participate in the decondensation of sperm nuclei and the maintenance and protection of non-condensed chromatin.

Journal

Aquaculture International – Springer Journals

Published: Apr 1, 2022

Keywords: Add1; Cytoskeleton; Eriocheir sinensis; Spermatogenesis; Decondensed sperm nuclei

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