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- Publisher
- Oxford University Press
- Copyright
- Copyright © 1985 by The Endocrine Society
- ISSN
- 0013-7227
- eISSN
- 1945-7170
- DOI
- 10.1210/endo-116-4-1666
- Publisher site
- See Article on Publisher Site
Abstract
Although treatment of cultured granulosa cells with gonadotropins increases their fibrinolytic activity, the biochemical nature of this effect is unclear. We have used sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and fibrin autography techniques to characterize the fibrinolytic components secreted by granulosa cells. The fibrinolytic activity of these cells results from the production of both a tissue-type plasminogen activator (t-PA) and a urokinase-like activator (u-PA). The cells also produce an inhibitor of fibrinolysis (antiactivator). FSH and LH stimulate t-PA activity and suppress antiactivator activity, while u-PA activity is not affected by the gonadotropins. The differential regulation of these molecules by the gonadotropins may be essential for ovulation. This content is only available as a PDF. Author notes 3 TO whom requests for reprints should be addressed. Copyright © 1985 by The Endocrine Society
Journal
Endocrinology – Oxford University Press
Published: Apr 1, 1985