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References (32)
-
C. Chung, R. Coolbaugh (1986)
ent-Kaurene Biosynthesis in Cell-Free Extracts of Excised Parts of Tall and Dwarf Pea Seedlings.Plant physiology, 80 2
-
John Duncan, C. West (1981)
Properties of Kaurene Synthetase from Marah macrocarpus Endosperm: Evidence for the Participation of Separate but Interacting Enzymes.Plant physiology, 68 5
-
Christopher Mau, Charles West (1994)
Cloning of casbene synthase cDNA: evidence for conserved structural features among terpenoid cyclases in plants.Proceedings of the National Academy of Sciences of the United States of America, 91 18
-
G. Bartley, P. Viitanen, K. Bacot, P. Scolnik (1992)
A tomato gene expressed during fruit ripening encodes an enzyme of the carotenoid biosynthesis pathway.The Journal of biological chemistry, 267 8
-
R. Bensen, G. Johal, V. Crane, J. Tossberg, P. Schnable, R. Meeley, S. Briggs (1995)
Cloning and characterization of the maize An1 gene.The Plant cell, 7
-
R. LaFever, B. Vogel, R. Croteau (1994)
Diterpenoid resin acid biosynthesis in conifers: enzymatic cyclization of geranylgeranyl pyrophosphate to abietadiene, the precursor of abietic acid.Archives of biochemistry and biophysics, 313 1
-
K. Keegstra, L. Olsen, S. Theg (1989)
Chloroplastic Precursors and their Transport Across the Envelope Membranes, 40
-
Joseph Chappell (1995)
The Biochemistry and Molecular Biology of Isoprenoid Metabolism, 107
-
Tai-ping Sun, Yuji Kamiya (1994)
The Arabidopsis GA1 locus encodes the cyclase ent-kaurene synthetase A of gibberellin biosynthesis.The Plant cell, 6 10
-
I. Railton, Bruce Fellows, C. West (1984)
Ent-kaurene synthesis in chloroplasts from higher plantsPhytochemistry, 23
-
J. Graebe, P. Hedden, P. Gaskin, J. Macmillan (1974)
Biosynthesis of gibberellins A12, A15, A24, A36, and A37 by a cell-free system from Cucurbita maximaPhytochemistry, 13
-
Jean Edwards, J. Delort, J. Mallet (1991)
Oligodeoxyribonucleotide ligation to single-stranded cDNAs: a new tool for cloning 5' ends of mRNAs and for constructing cDNA libraries by in vitro amplification.Nucleic acids research, 19 19
-
M. Frohman, M. Dush, G. Martin (1988)
Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.Proceedings of the National Academy of Sciences of the United States of America, 85 23
-
A. Phillips, D. Ward, S. Uknes, N. Appleford, T. Lange, A. Huttly, P. Gaskin, J. Graebe, P. Hedden (1995)
Isolation and Expression of Three Gibberellin 20-Oxidase cDNA Clones from Arabidopsis, 108
-
R. Fray, Andrew Wallace, P. Fraser, D. Valero, P. Hedden, P. Bramley, D. Grierson (1995)
Constitutive expression of a fruit phytoene synthase gene in transgenic tomatoes causes dwarfism by redirecting metabolites from the gibberellin pathwayPlant Journal, 8
-
E. Amann, Jürgen Brosius (1985)
"ATG vectors' for regulated high-level expression of cloned genes in Escherichia coli.Gene, 40 2-3
-
J. Graebe (1987)
Gibberellin Biosynthesis and ControlAnnual Review of Plant Biology, 38
-
Tai-ping Sun, H. Goodman, F. Ausubel (1992)
Cloning the Arabidopsis GA1 Locus by Genomic Subtraction.The Plant cell, 4
-
J. Zeevaart, D. Gage (1993)
ent-Kaurene Biosynthesis Is Enhanced by Long Photoperiods in the Long-Day Plants Spinacia oleracea L. and Agrostemma githago L, 101
-
Takuma Miki, Y. Kamiya, M. Fukazawa, Tadashi Ichikawa, A. Sakurai (1990)
Sites of inhibition by a plant-growth regulator, 4′-chloro-2′-(α-hydroxybenzyl)isonicotinanilide(inabenfide), and its related compounds in the biosynthesis of gibberellins.Plant and Cell Physiology, 31
-
C. Spickett, K. Ponnamperuma, C. Abell (1994)
The resolution of diterpene cyclase activities from Ricinus communisPhytochemistry, 37
-
T. Lange, P. Hedden, J. Graebe (1994)
Expression cloning of a gibberellin 20-oxidase, a multifunctional enzyme involved in gibberellin biosynthesis.Proceedings of the National Academy of Sciences of the United States of America, 91 18
-
M. Kuntz, S. Römer, C. Suire, P. Hugueney, J. Weil, R. Schantz, B. Camara (1992)
Identification of a cDNA for the plastid-located geranylgeranyl pyrophosphate synthase from Capsicum annuum: correlative increase in enzyme activity and transcript level during fruit ripening.The Plant journal : for cell and molecular biology, 2 1
-
Paul Ecklund, T. Moore (1974)
Correlations of Growth Rate and De-etiolation with Rate of Ent-Kaurene Biosynthesis in Pea (Pisum sativum L.).Plant physiology, 53 1
-
P. Scolnik, G. Bartley (1994)
Nucleotide Sequence of an Arabidopsis cDNA for Geranylgeranyl Pyrophosphate Synthase, 104
-
Y. Xu, L. Li, K. Wu, A. Peeters, D. Gage, J. Zeevaart (1995)
The GA5 locus of Arabidopsis thaliana encodes a multifunctional gibberellin 20-oxidase: molecular cloning and functional expression.Proceedings of the National Academy of Sciences of the United States of America, 92 14
-
S. Colby, W. Alonso, E. Katahira, D. McGarvey, R. Croteau (1993)
4S-limonene synthase from the oil glands of spearmint (Mentha spicata). cDNA isolation, characterization, and bacterial expression of the catalytically active monoterpene cyclase.The Journal of biological chemistry, 268 31
-
T. Saito, Hiroshi Abe, Hisakazu Yamane, Akira Sakurai, N. Murofushi, K. Takio, Nobutaka Takahashi, Yuji Kamiya (1995)
Purification and Properties of ent-Kaurene Synthase B from Immature Seeds of Pumpkin, 109
-
H. Chiang, I. Hwang, H. Goodman (1995)
Isolation of the Arabidopsis GA4 locus.The Plant cell, 7
-
Peter Facchini, Joseph Chappell (1992)
Gene family for an elicitor-induced sesquiterpene cyclase in tobacco.Proceedings of the National Academy of Sciences of the United States of America, 89 22
-
Chu Guana, Ping Lib, Paul Riggsa, Hiroshi Inouyeb (1988)
Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein.Gene, 67
-
R. Winkler, T. Helentjaris (1995)
The maize Dwarf3 gene encodes a cytochrome P450-mediated early step in Gibberellin biosynthesis.The Plant cell, 7
- Publisher
- Wiley
- Copyright
- Copyright © 1996 Wiley Subscription Services, Inc., A Wiley Company
- ISSN
- 0960-7412
- eISSN
- 1365-313X
- DOI
- 10.1046/j.1365-313X.1996.10020203.x
- Publisher site
- See Article on Publisher Site
Abstract
The first committed step in the formation of diterpenoids leading to gibberellin (GA) biosynthesis is the conversion of geranylgeranyl diphosphate (GGDP) to ent‐kaurene. ent‐Kaurene synthase A (KSA) catalyzes the conversion of GGDP to copalyl diphosphate (CDP), which is subsequently converted to ent‐kaurene by ent‐kaurene synthase B (KSB). A full‐length KSB cDNA was isolated from developing cotyledons in immature seeds of pumpkin (Cucurbita maxima L.). Degenerate oligonucleotide primers were designed from the amino acid sequences obtained from the purified protein to amplify a cDNA fragment, which was used for library screening. The isolated full‐length cDNA was expressed in Escherichia coli as a fusion protein, which demonstrated the KSB activity to cyclize [3H]CDP to [3H]ent‐kaurene. The KSB transcript was most abundant in growing tissues, but was detected in every organ in pumpkin seedlings. The deduced amino acid sequence shares significant homology with other terpene cyclases, including the conserved DDXXD motif, a putative divalent metal ion‐diphosphate complex binding site. A putative transit peptide sequence that may target the translated product into the plastids is present in the N‐terminal region.
Journal
The Plant Journal – Wiley
Published: Aug 1, 1996