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Identification and characterization of Saccharomyces cerevisiae mutants defective in fluid‐phase endocytosis

Identification and characterization of Saccharomyces cerevisiae mutants defective in fluid‐phase... A mutant library generated by the European Functional Analysis Network (EUROFAN) was screened for strains defective in fluid‐phase endocytosis. Accumulation of Lucifer yellow in the vacuole was used as a marker for efficient endocytosis. Fourteen mutants, including ede1Δ, rcy1Δ, sys1Δ and tlg2Δ, previously described to be involved in membrane trafficking, were identified in this screen. α‐Factor uptake, endocytosis of FM4‐64, carboxypeptidase Y secretion, vacuolar morphology, and a vma2 synthetic growth defect were used as criteria to characterize the endocytic defect of the mutant strains obtained. Accordingly, eight mutant strains have endocytic phenotypes in addition to their defect in Lucifer yellow accumulation. These fluid‐phase endocytosis mutants are defective at different steps of the endocytic pathway. Interestingly, only two mutants were defective for internalization, two for vacuolar protein sorting and four mutants had aberrant vacuolar morphologies. Some of the mutants identified in this screen that sort carboxypeptidase Y correctly may affect endocytosis at an early post‐internalization step before the intersection of the endocytic with the vacuolar protein‐sorting pathway. Copyright © 2001 John Wiley & Sons, Ltd. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Yeast Wiley

Identification and characterization of Saccharomyces cerevisiae mutants defective in fluid‐phase endocytosis

Wiederkehr, Andreas; Meier, Karsten D.; Riezman, Howard
Yeast , Volume 18 (8) – Jan 15, 2001
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References (49)

Publisher
Wiley
Copyright
Copyright © 2001 Wiley Subscription Services
ISSN
0749-503X
eISSN
1097-0061
DOI
10.1002/yea.726
pmid
11378903
Publisher site
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Abstract

A mutant library generated by the European Functional Analysis Network (EUROFAN) was screened for strains defective in fluid‐phase endocytosis. Accumulation of Lucifer yellow in the vacuole was used as a marker for efficient endocytosis. Fourteen mutants, including ede1Δ, rcy1Δ, sys1Δ and tlg2Δ, previously described to be involved in membrane trafficking, were identified in this screen. α‐Factor uptake, endocytosis of FM4‐64, carboxypeptidase Y secretion, vacuolar morphology, and a vma2 synthetic growth defect were used as criteria to characterize the endocytic defect of the mutant strains obtained. Accordingly, eight mutant strains have endocytic phenotypes in addition to their defect in Lucifer yellow accumulation. These fluid‐phase endocytosis mutants are defective at different steps of the endocytic pathway. Interestingly, only two mutants were defective for internalization, two for vacuolar protein sorting and four mutants had aberrant vacuolar morphologies. Some of the mutants identified in this screen that sort carboxypeptidase Y correctly may affect endocytosis at an early post‐internalization step before the intersection of the endocytic with the vacuolar protein‐sorting pathway. Copyright © 2001 John Wiley & Sons, Ltd.

Journal

Yeast – Wiley

Published: Jan 15, 2001

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