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Preparation and characterisation of an antiserum to the mouse candidate for immunoglobulin D

Preparation and characterisation of an antiserum to the mouse candidate for immunoglobulin D ANALYSIS of mouse B-lymphocyte surface immunoglobulin (Ig) by isotopic labelling techniques reveals little, if any, IgA or IgG. Instead, the surface Ig consists of monomeric IgM and an Ig similar in physicochemical properties to human IgD1–3. In the absence of sequence comparison between human IgD and its assumed mouse counterpart, this assignment is tentative. With this proviso, however, and for the sake of clarity in presentation, we refer to this immunoglobulin as IgD. During development, IgM-bearing cells are the first to arise and the ratio of IgM–IgD determined with labelled adult lymphocytes is higher in the spleen than in lymph nodes or Peyer's patches1,4. These findings, however, give the total yield of the two Ig classes and not their distribution on individual lymphocytes. On the basis of the absence of immunoglobulins other than IgM or IgD on the surface of B lymphocytes, we indicated the presence of cells bearing IgM or IgD, or IgM and IgD in mouse spleen cell suspensions5,6. In these experiments, IgM was first capped with rhodamine-labelled anti-μ and subsequent ring staining with fluorescein-labelled anti-Fab in the presence of sodium azide was taken as evidence for the presence of IgD. We report here the preparation of an antibody specific for mouse IgD, and its use in the characterisation of mouse B-lymphocyte surface Ig. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nature Springer Journals

Preparation and characterisation of an antiserum to the mouse candidate for immunoglobulin D

ABNEY, ERIKA R.; HUNTER, I. R.; PARKHOUSE, R. M. E.
Nature , Volume 259 (5542) – Feb 5, 1976
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References (18)

Publisher
Springer Journals
Copyright
Copyright © 1976 by Nature Publishing Group
Subject
Science, Humanities and Social Sciences, multidisciplinary; Science, Humanities and Social Sciences, multidisciplinary; Science, multidisciplinary
ISSN
0028-0836
eISSN
1476-4687
DOI
10.1038/259404a0
Publisher site
See Article on Publisher Site

Abstract

ANALYSIS of mouse B-lymphocyte surface immunoglobulin (Ig) by isotopic labelling techniques reveals little, if any, IgA or IgG. Instead, the surface Ig consists of monomeric IgM and an Ig similar in physicochemical properties to human IgD1–3. In the absence of sequence comparison between human IgD and its assumed mouse counterpart, this assignment is tentative. With this proviso, however, and for the sake of clarity in presentation, we refer to this immunoglobulin as IgD. During development, IgM-bearing cells are the first to arise and the ratio of IgM–IgD determined with labelled adult lymphocytes is higher in the spleen than in lymph nodes or Peyer's patches1,4. These findings, however, give the total yield of the two Ig classes and not their distribution on individual lymphocytes. On the basis of the absence of immunoglobulins other than IgM or IgD on the surface of B lymphocytes, we indicated the presence of cells bearing IgM or IgD, or IgM and IgD in mouse spleen cell suspensions5,6. In these experiments, IgM was first capped with rhodamine-labelled anti-μ and subsequent ring staining with fluorescein-labelled anti-Fab in the presence of sodium azide was taken as evidence for the presence of IgD. We report here the preparation of an antibody specific for mouse IgD, and its use in the characterisation of mouse B-lymphocyte surface Ig.

Journal

Nature – Springer Journals

Published: Feb 5, 1976

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