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Development of a loop‐mediated isothermal amplification assay for specific detection of all known subspecies of Clavibacter michiganensis

Development of a loop‐mediated isothermal amplification assay for specific detection of all known... Clavibacter michiganensis, diagnostics, Aims: Clavibacter michiganensis is an important bacterial plant pathogen that isothermal amplification, LAMP, plant pathogenic bacteria, surveillance. causes vast destruction to agriculturally important crops worldwide. Early detection is critical to evaluate disease progression and to implement efficient Correspondence control measures to avoid serious epidemics. In this study, we developed a Mohammad Arif, Department of Plant and sensitive, specific and robust loop-mediated isothermal amplification (LAMP) Environmental Protection Sciences, 3190 assay for detection of all known subspecies of C. michiganensis. Maile Way, St John 312, University of Hawaii Methods and Results: Whole genome comparative genomics approach was at Manoa, Honolulu, HI 96822, USA. taken to identify a unique and conserved region within all known subspecies of E-mail: [email protected] C. michiganensis. Primer specificity was evaluated in silico and with 64 bacterial 2018/1414: received 19 July 2018, revised 3 strains included in inclusivity and exclusivity panels; no false positives or false October 2018 and accepted 5 October 2018 negatives were detected. Both the sensitivity and spiked assay of the developed LAMP assay was 1 fg of the pathogen DNA per reaction. A 100% accuracy was doi:10.1111/jam.14128 observed when tested with infected plant samples. Conclusions: The developed LAMP assay is simple, http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Applied Microbiology Oxford University Press

Development of a loop‐mediated isothermal amplification assay for specific detection of all known subspecies of Clavibacter michiganensis

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References (68)

Publisher
Oxford University Press
Copyright
Copyright © 2019 Wiley Subscription Services, Inc., A Wiley Company
ISSN
1364-5072
eISSN
1365-2672
DOI
10.1111/jam.14128
Publisher site
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Abstract

Clavibacter michiganensis, diagnostics, Aims: Clavibacter michiganensis is an important bacterial plant pathogen that isothermal amplification, LAMP, plant pathogenic bacteria, surveillance. causes vast destruction to agriculturally important crops worldwide. Early detection is critical to evaluate disease progression and to implement efficient Correspondence control measures to avoid serious epidemics. In this study, we developed a Mohammad Arif, Department of Plant and sensitive, specific and robust loop-mediated isothermal amplification (LAMP) Environmental Protection Sciences, 3190 assay for detection of all known subspecies of C. michiganensis. Maile Way, St John 312, University of Hawaii Methods and Results: Whole genome comparative genomics approach was at Manoa, Honolulu, HI 96822, USA. taken to identify a unique and conserved region within all known subspecies of E-mail: [email protected] C. michiganensis. Primer specificity was evaluated in silico and with 64 bacterial 2018/1414: received 19 July 2018, revised 3 strains included in inclusivity and exclusivity panels; no false positives or false October 2018 and accepted 5 October 2018 negatives were detected. Both the sensitivity and spiked assay of the developed LAMP assay was 1 fg of the pathogen DNA per reaction. A 100% accuracy was doi:10.1111/jam.14128 observed when tested with infected plant samples. Conclusions: The developed LAMP assay is simple,

Journal

Journal of Applied Microbiology – Oxford University Press

Published: Jan 1, 2019

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