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Effect of surgical activation on ovarian functionality and follicular reactivation in ovine animal model. pilot study

Effect of surgical activation on ovarian functionality and follicular reactivation in ovine... ii6 | Abstracts 1 1 2,3,4,5 1,6,7 8,9 1,6,7 Jesús Usón Minimally Invasive Surgery Centre, Cáceres 10004, Spain CMEMS-UMinho, University of Minho, Guimarães 4800-058, Portugal LABBELS-Associate Laboratory, Braga/Guimarães, Portugal CF-UM-UP, Physics Centre of Minho and Porto Universities, University of Minho, Campus de Gualtar, Braga 4710-057, Portugal IB-S, Institute of Science and Innovation for Bio-Sustainability, University of Minho, Campus de Gualtar, Braga 4710-057, Portugal Abstract citation ID: znae322.023 Centro de Investigación Biomédica en Red de Enfermedades Carviovasculares Effect of surgical activation on ovarian functionality and (CIBERCV), Instituto de Salud Carlos III, Madrid 28029, Spain follicular reactivation in ovine animal model. pilot study RICORS-TERAV Network, Instituto de Salud Carlos III, Madrid 28029, Spain BCMaterials, Basque Center for Materials, Applications and Nanostructures, 1 2 3 4 S. Sánchez-Mateos , F. Fabregues , E. Párraga-Ros , C. Herranz-DIez , UPV/EHU Science Park, 48940 Leioa, Spain 4 5 5 1 J. Otero , I.S. Alvarez , J.A. Domínguez , F.M. Sánchez Margallo Ikerbasque, Basque Foundation for Science, 48009 Bilbao, Spain Assisted Reproduction Unit. Jesús Usón Minimally Invasive Surgery Center. Caceres. Spain Institute of Gynecology, Obstetrics and Neonatology (ICGON). Hospital Clinic of Barcelona. Barcelona, Spain Department of Anatomy and Comparative Pathology. Veterinary school. University of Murcia. Spain Biophysics and Bioengineering Unit, Faculty of Medicine and Health Sciences, University of Barcelona, Barcelona, Spain Extremadura Institute of Assisted Reproduction IERA Quironsalud. Badajoz. Spain Introduction: Poor ovarian reserve (POI) is one of the main factors that affect women with fertility problems. In this study, we tested whether making small incisions in the ovarian cortex leads to changes in the biomechanics of the ovary that could result in reactivation of ovarian functionality and folliculogenesis. Methods: Given its similarity to the female reproductive system, both anatomically and physiologically, we used 5-year-old sheep (n=3) as an animal model. Incisions were made in one of the ovaries (OD) by laparoscopy, with the other ovary left as the control (OC). To study the effect in the short, medium, and long term, oophorectomy of both ovaries was performed at 2, 4, and 6 months, respectively, with one sheep per group. Half of each ovary was fixed in 4% paraformaldehyde for histological study, a small fragment was fixed in OCT for the biomechanics study, and the remainder was used to measure the expression of the HIPO pathway genes involved in follicular reactivation. Results: The data from the histological analysis show that the number of growing follicles was greater at 2 months, with a higher proportion of growing follicles in the operated ovary than in the control ovary (58.9% vs. 29.7%, respectively). The biomechanical study of the cortex using AFM showed a similar trend, with greater stiffness at 2 months. Gene expression is currently under analysis. Conclusion: Surgical manipulation of the ovary resulted in short-term reactivation of folliculogenesis. 11 2 33 4 Fundación Centro de Cirugía de Mínima Invasión Jesús Usón (CCMIJU) 11 1 1 1 Centro de Visión por Computador (CVC-UAB) 23 3 Grupo de Sistemas Informáticos de Nueva Generación (SING) – Universidade de Department of Biochemistry and Molecular Biology, Faculty of Medicine, Vigo 4 Complutense University of Madrid, Madrid Grupo de Oncología Digestiva de Ourense (GIODO) - Servizo Galego de Saude Department of Physiology, Faculty of Medicine, Complutense University of Madrid, Madrid Department of Surgery, and Radiology and Physical Medicine, Faculty of Medicine and Nursing, University of the Basque Country, Bilbao http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png British Journal of Surgery Oxford University Press

Effect of surgical activation on ovarian functionality and follicular reactivation in ovine animal model. pilot study

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Oxford University Press
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© The Author(s) 2025. Published by Oxford University Press on behalf of BJS Foundation Ltd. All rights reserved. For commercial re-use, please contact [email protected] for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact [email protected].
ISSN
0007-1323
eISSN
1365-2168
DOI
10.1093/bjs/znae322.023
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Abstract

ii6 | Abstracts 1 1 2,3,4,5 1,6,7 8,9 1,6,7 Jesús Usón Minimally Invasive Surgery Centre, Cáceres 10004, Spain CMEMS-UMinho, University of Minho, Guimarães 4800-058, Portugal LABBELS-Associate Laboratory, Braga/Guimarães, Portugal CF-UM-UP, Physics Centre of Minho and Porto Universities, University of Minho, Campus de Gualtar, Braga 4710-057, Portugal IB-S, Institute of Science and Innovation for Bio-Sustainability, University of Minho, Campus de Gualtar, Braga 4710-057, Portugal Abstract citation ID: znae322.023 Centro de Investigación Biomédica en Red de Enfermedades Carviovasculares Effect of surgical activation on ovarian functionality and (CIBERCV), Instituto de Salud Carlos III, Madrid 28029, Spain follicular reactivation in ovine animal model. pilot study RICORS-TERAV Network, Instituto de Salud Carlos III, Madrid 28029, Spain BCMaterials, Basque Center for Materials, Applications and Nanostructures, 1 2 3 4 S. Sánchez-Mateos , F. Fabregues , E. Párraga-Ros , C. Herranz-DIez , UPV/EHU Science Park, 48940 Leioa, Spain 4 5 5 1 J. Otero , I.S. Alvarez , J.A. Domínguez , F.M. Sánchez Margallo Ikerbasque, Basque Foundation for Science, 48009 Bilbao, Spain Assisted Reproduction Unit. Jesús Usón Minimally Invasive Surgery Center. Caceres. Spain Institute of Gynecology, Obstetrics and Neonatology (ICGON). Hospital Clinic of Barcelona. Barcelona, Spain Department of Anatomy and Comparative Pathology. Veterinary school. University of Murcia. Spain Biophysics and Bioengineering Unit, Faculty of Medicine and Health Sciences, University of Barcelona, Barcelona, Spain Extremadura Institute of Assisted Reproduction IERA Quironsalud. Badajoz. Spain Introduction: Poor ovarian reserve (POI) is one of the main factors that affect women with fertility problems. In this study, we tested whether making small incisions in the ovarian cortex leads to changes in the biomechanics of the ovary that could result in reactivation of ovarian functionality and folliculogenesis. Methods: Given its similarity to the female reproductive system, both anatomically and physiologically, we used 5-year-old sheep (n=3) as an animal model. Incisions were made in one of the ovaries (OD) by laparoscopy, with the other ovary left as the control (OC). To study the effect in the short, medium, and long term, oophorectomy of both ovaries was performed at 2, 4, and 6 months, respectively, with one sheep per group. Half of each ovary was fixed in 4% paraformaldehyde for histological study, a small fragment was fixed in OCT for the biomechanics study, and the remainder was used to measure the expression of the HIPO pathway genes involved in follicular reactivation. Results: The data from the histological analysis show that the number of growing follicles was greater at 2 months, with a higher proportion of growing follicles in the operated ovary than in the control ovary (58.9% vs. 29.7%, respectively). The biomechanical study of the cortex using AFM showed a similar trend, with greater stiffness at 2 months. Gene expression is currently under analysis. Conclusion: Surgical manipulation of the ovary resulted in short-term reactivation of folliculogenesis. 11 2 33 4 Fundación Centro de Cirugía de Mínima Invasión Jesús Usón (CCMIJU) 11 1 1 1 Centro de Visión por Computador (CVC-UAB) 23 3 Grupo de Sistemas Informáticos de Nueva Generación (SING) – Universidade de Department of Biochemistry and Molecular Biology, Faculty of Medicine, Vigo 4 Complutense University of Madrid, Madrid Grupo de Oncología Digestiva de Ourense (GIODO) - Servizo Galego de Saude Department of Physiology, Faculty of Medicine, Complutense University of Madrid, Madrid Department of Surgery, and Radiology and Physical Medicine, Faculty of Medicine and Nursing, University of the Basque Country, Bilbao

Journal

British Journal of Surgery – Oxford University Press

Published: Jan 22, 2025

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