Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 7-Day Trial for You or Your Team.

Learn More →

ETN‐1: A new human endometrial carcinoma cell line producing ascites and distant metastases in nude mice

ETN‐1: A new human endometrial carcinoma cell line producing ascites and distant metastases in... A human carcinoma cell line (ETN‐I) has been established from a skin metastasis of a moderately differentiated adenocarcinoma of endometrial origin. The cell line has been so far maintained for 27 months through 55 passages, growing as a monolayer as well as in 3‐dimensional clusters with a population doubling time of 72 hr. The number of chromosomes per cell varied from 39 to 107 (average number 61.0 ± 19.8), with a modal number of 46–48. Seven clonal marker chromosomes were detected. Flow cytometric analysis revealed a population of pseudo‐tetraploid cells (DNA index 2.1) next to a pseudo‐diploid population (DNA index 1.1). The epithelial character of the cells was confirmed by a positive immunocytochemical reaction using monoclonal antibodies (MAbs) to different keratins, the epithelial cell markers BW 495/36 and HMFG‐2, as well as by the presence of many junctional complexes. The tumour cells retained a positive reaction with the anti‐ovarian carcinoma OV‐TL 3, OV‐TL 10 and OC 125 MAbs, although the reaction was markedly diminished in comparison with the original tumour. Tumour cells inoculated subcutaneously in nude mice produced well differentiated adenomatous tumour nodules with formation of glandular lumina and basal lamina. Tumour cells injected intraperitoneally produced malignant ascites and regional as well as distant metastases of adenomatous character. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png International Journal of Cancer Wiley

ETN‐1: A new human endometrial carcinoma cell line producing ascites and distant metastases in nude mice

Download PDF
6 pages

Loading next page...
 
/lp/wiley/etn-1-a-new-human-endometrial-carcinoma-cell-line-producing-ascites-zdTPXp3Hhm

References (35)

Publisher
Wiley
Copyright
Copyright © 1989 Wiley‐Liss, Inc., A Wiley Company
ISSN
0020-7136
eISSN
1097-0215
DOI
10.1002/ijc.2910430624
Publisher site
See Article on Publisher Site

Abstract

A human carcinoma cell line (ETN‐I) has been established from a skin metastasis of a moderately differentiated adenocarcinoma of endometrial origin. The cell line has been so far maintained for 27 months through 55 passages, growing as a monolayer as well as in 3‐dimensional clusters with a population doubling time of 72 hr. The number of chromosomes per cell varied from 39 to 107 (average number 61.0 ± 19.8), with a modal number of 46–48. Seven clonal marker chromosomes were detected. Flow cytometric analysis revealed a population of pseudo‐tetraploid cells (DNA index 2.1) next to a pseudo‐diploid population (DNA index 1.1). The epithelial character of the cells was confirmed by a positive immunocytochemical reaction using monoclonal antibodies (MAbs) to different keratins, the epithelial cell markers BW 495/36 and HMFG‐2, as well as by the presence of many junctional complexes. The tumour cells retained a positive reaction with the anti‐ovarian carcinoma OV‐TL 3, OV‐TL 10 and OC 125 MAbs, although the reaction was markedly diminished in comparison with the original tumour. Tumour cells inoculated subcutaneously in nude mice produced well differentiated adenomatous tumour nodules with formation of glandular lumina and basal lamina. Tumour cells injected intraperitoneally produced malignant ascites and regional as well as distant metastases of adenomatous character.

Journal

International Journal of Cancer – Wiley

Published: Jun 15, 1989

There are no references for this article.