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Development of an aqueous temperature-indicating technique and its application to clinical laboratory instrumentation.

Development of an aqueous temperature-indicating technique and its application to clinical... Abstract We describe a new technique for monitoring intra-cuvette temperatures in temperature-controlled spectrophotometric cells by use of two solutions, one for which absorbance is highly sensitive to temperature and one for which absorbance is insensitive to temperature. The first solution, cresol red in tris(hydroxymethyl)aminomethane buffer, is used to construct an absorbance-vs.-temperature calibration curvae. The second solution, cresol red in sodium hydroxide, is used for optical calibration of the spectrophotometric system to be tested. The combination of these two procedures allows accurate estimation of temperature. We show how this technique is applied to temperature measurement in several types of laboratory instrumentation. This content is only available as a PDF. © 1976 The American Association for Clinical Chemistry, Inc. This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model) http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Clinical Chemistry Oxford University Press

Development of an aqueous temperature-indicating technique and its application to clinical laboratory instrumentation.

Bowie,, L; Esters,, F; Bolin,, J; Gochman,, N
Clinical Chemistry , Volume 22 (4) – Apr 1, 1976
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References (4)

Publisher
Oxford University Press
Copyright
© 1976 The American Association for Clinical Chemistry, Inc.
ISSN
0009-9147
eISSN
1530-8561
DOI
10.1093/clinchem/22.4.449
Publisher site
See Article on Publisher Site

Abstract

Abstract We describe a new technique for monitoring intra-cuvette temperatures in temperature-controlled spectrophotometric cells by use of two solutions, one for which absorbance is highly sensitive to temperature and one for which absorbance is insensitive to temperature. The first solution, cresol red in tris(hydroxymethyl)aminomethane buffer, is used to construct an absorbance-vs.-temperature calibration curvae. The second solution, cresol red in sodium hydroxide, is used for optical calibration of the spectrophotometric system to be tested. The combination of these two procedures allows accurate estimation of temperature. We show how this technique is applied to temperature measurement in several types of laboratory instrumentation. This content is only available as a PDF. © 1976 The American Association for Clinical Chemistry, Inc. This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)

Journal

Clinical Chemistry – Oxford University Press

Published: Apr 1, 1976

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