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Regulation of expression of the cell adhesion receptors, integrins, by recombinant human interleukin‐1β in human osteosarcoma cells: Inhibition of cell proliferation and stimulation of alkaline phosphatase activity

Regulation of expression of the cell adhesion receptors, integrins, by recombinant human... 10.1002/jcp.1041380210.abs Recombinant human interleukin‐1β, a mediator of osteoblastic cell function, was found to regulate the expression of the cell adhesion receptors, integrins, on human osteosarcoma cells. Interleukin‐1β (IL‐1β) at picomolar concentrations, specifically elevated approximately six‐ to tenfold the expression of the β1 subunit and its associated α subunits, but not the related vitronectin receptor, within 20 hours. Integrin β1 messenger RNA levels were elevated within 6 hours and peaked to tenfold higher levels after 20 hours exposure to IL‐1β in two human osteosarcoma cell lines. The increase in the cell‐surface β1 integrins resulted in a stronger binding of the IL‐1β‐treated cells to fibronectin. Cell growth was also inhibited by IL‐1β cell morphology was altered, and IL‐1β‐treated cells expressed an approximately two‐ to threefold higher alkaline phosphatase. This increase in alkaline phosphatase activity was found to be independent of the inhibition of cell proliferation. These data indicate that the β1 integrin family of cell surface receptors is a target for regulation by IL‐1β which also regulates cell proliferation and the expression of the osteoblastic phenotype in human osteosarcoma cells. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Cellular Physiology Wiley

Regulation of expression of the cell adhesion receptors, integrins, by recombinant human interleukin‐1β in human osteosarcoma cells: Inhibition of cell proliferation and stimulation of alkaline phosphatase activity

Dedhar, Shoukat
Journal of Cellular Physiology , Volume 138 (2) – Feb 1, 1989
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References (48)

Publisher
Wiley
Copyright
Copyright © 1989 Wiley‐Liss, Inc.
ISSN
0021-9541
eISSN
1097-4652
DOI
10.1002/jcp.1041380210
pmid
2521862
Publisher site
See Article on Publisher Site

Abstract

10.1002/jcp.1041380210.abs Recombinant human interleukin‐1β, a mediator of osteoblastic cell function, was found to regulate the expression of the cell adhesion receptors, integrins, on human osteosarcoma cells. Interleukin‐1β (IL‐1β) at picomolar concentrations, specifically elevated approximately six‐ to tenfold the expression of the β1 subunit and its associated α subunits, but not the related vitronectin receptor, within 20 hours. Integrin β1 messenger RNA levels were elevated within 6 hours and peaked to tenfold higher levels after 20 hours exposure to IL‐1β in two human osteosarcoma cell lines. The increase in the cell‐surface β1 integrins resulted in a stronger binding of the IL‐1β‐treated cells to fibronectin. Cell growth was also inhibited by IL‐1β cell morphology was altered, and IL‐1β‐treated cells expressed an approximately two‐ to threefold higher alkaline phosphatase. This increase in alkaline phosphatase activity was found to be independent of the inhibition of cell proliferation. These data indicate that the β1 integrin family of cell surface receptors is a target for regulation by IL‐1β which also regulates cell proliferation and the expression of the osteoblastic phenotype in human osteosarcoma cells.

Journal

Journal of Cellular Physiology – Wiley

Published: Feb 1, 1989

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