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Integration of whole‐genome DNA methylation data with RNA sequencing data to identify markers for bull fertility

Integration of whole‐genome DNA methylation data with RNA sequencing data to identify markers for... Predicting bull fertility prior to breeding is a current challenge for the dairy industry. The use of molecular biomarkers has been previously assessed. However, the integration of this information has not been performed to extract biologically relevant markers. The goal of this study was to integrate DNA methylation data with previously published RNA‐sequencing results in order to identify candidate markers for sire fertility. A total of 1765 differentially methylated cytosines were found between high‐ and low‐fertility sires. Ten genes associated with 11 differentially methylated cytosines were found in a previous study of gene expression between high‐ and low‐fertility sires. Additionally, two of these genes code for proteins found exclusively in bull seminal plasma. Collectively, our results reveal 10 genes that could be used in the future as a panel for predicting bull fertility. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Animal Genetics Wiley

Integration of whole‐genome DNA methylation data with RNA sequencing data to identify markers for bull fertility

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References (74)

Publisher
Wiley
Copyright
Copyright © 2020 Stichting International Foundation for Animal Genetics
ISSN
0268-9146
eISSN
1365-2052
DOI
10.1111/age.12941
Publisher site
See Article on Publisher Site

Abstract

Predicting bull fertility prior to breeding is a current challenge for the dairy industry. The use of molecular biomarkers has been previously assessed. However, the integration of this information has not been performed to extract biologically relevant markers. The goal of this study was to integrate DNA methylation data with previously published RNA‐sequencing results in order to identify candidate markers for sire fertility. A total of 1765 differentially methylated cytosines were found between high‐ and low‐fertility sires. Ten genes associated with 11 differentially methylated cytosines were found in a previous study of gene expression between high‐ and low‐fertility sires. Additionally, two of these genes code for proteins found exclusively in bull seminal plasma. Collectively, our results reveal 10 genes that could be used in the future as a panel for predicting bull fertility.

Journal

Animal GeneticsWiley

Published: Aug 1, 2020

Keywords: ; ; ;

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