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Relative Proportions of Pathogen‐Related Oral Spirochetes (PROS) and Treponema denticola in Supragingival and Subgingival Plaque From Patients With Periodontitis

Relative Proportions of Pathogen‐Related Oral Spirochetes (PROS) and Treponema denticola in... The purpose of this study was to use monoclonal antibodies to enumerate spirochetes in dental plaque, including the newly recognized pathogen‐related oral spirochete (PROS) and specific serovars of Treponema denticola. Plaque was collected from control subjects with no apparent periodontal disease and from sites of moderate to severe chronic periodontitis in patients with inflammatory periodontal disease. Individual monoclonal antibodies were used to determine whether spirochetes were present and then a doublestaining protocol was employed to count total spirochetes and specific treponemes in individual microscopic fields. Results indicate that spirochetes are more common at diseased sites and in subgingival plaque than at healthy sites or in supragingival plaque. Together PROS and T. denticola comprised the majority of all spirochetes in all samples and PROS and T. denticola serovars “B” and D were most numerous in plaque from patients with periodontitis. PROS were the majority of all spirochetes in supragingival plaque (76.2% ± 23.8%) and subgingival plaque (60.9% ± 19.1%) from periodontitis patients, significantly larger than the percentage of T. denticola serovar “B” (P <.001 for both supragingival and subgingival plaque) and serovar D (P <.01 for supragingival and P <.001 for subgingival plaque). These observations indicate that PROS are the predominant spirochete in plaque from sites of patients with periodontitis, but other analytical approaches are necessary to determine if PROS or T. denticola are pathogenic. J Periodontol 1992; 63:131–136. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Periodontology Wiley

Relative Proportions of Pathogen‐Related Oral Spirochetes (PROS) and Treponema denticola in Supragingival and Subgingival Plaque From Patients With Periodontitis

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References (12)

Publisher
Wiley
Copyright
© American Academy of Periodontology
ISSN
0022-3492
eISSN
1943-3670
DOI
10.1902/jop.1992.63.2.131
pmid
1372653
Publisher site
See Article on Publisher Site

Abstract

The purpose of this study was to use monoclonal antibodies to enumerate spirochetes in dental plaque, including the newly recognized pathogen‐related oral spirochete (PROS) and specific serovars of Treponema denticola. Plaque was collected from control subjects with no apparent periodontal disease and from sites of moderate to severe chronic periodontitis in patients with inflammatory periodontal disease. Individual monoclonal antibodies were used to determine whether spirochetes were present and then a doublestaining protocol was employed to count total spirochetes and specific treponemes in individual microscopic fields. Results indicate that spirochetes are more common at diseased sites and in subgingival plaque than at healthy sites or in supragingival plaque. Together PROS and T. denticola comprised the majority of all spirochetes in all samples and PROS and T. denticola serovars “B” and D were most numerous in plaque from patients with periodontitis. PROS were the majority of all spirochetes in supragingival plaque (76.2% ± 23.8%) and subgingival plaque (60.9% ± 19.1%) from periodontitis patients, significantly larger than the percentage of T. denticola serovar “B” (P <.001 for both supragingival and subgingival plaque) and serovar D (P <.01 for supragingival and P <.001 for subgingival plaque). These observations indicate that PROS are the predominant spirochete in plaque from sites of patients with periodontitis, but other analytical approaches are necessary to determine if PROS or T. denticola are pathogenic. J Periodontol 1992; 63:131–136.

Journal

Journal of Periodontology – Wiley

Published: Feb 1, 1992

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