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Changes in the electrochemical composition of cochlear fluids after intracisternal application of doxorubicin in the rat

Changes in the electrochemical composition of cochlear fluids after intracisternal application of... Since doxorubicin (Adriamycin) is known to bind on membranous negative surface charges, its effect on the electrochemical composition of the cochlear fluids was studied in rats. Doxorubicin was infused into the cerebrospinal fluid via the lateral cerebral ventricle. The endocochlear resting potential was recorded, and endolymph and perilymph of the scala vestibuli were collected from the basal cochlear turn before and 1, 2, and 4 h after the drug application. Na+, K+, and Cl− concentrations and osmolality of the endolymph and perilymph were measured in 1 nl aliquots. In perilymph, Cl− concentration increased 4 h after doxorubicin treatment to reach a concentration 8 mM higher than recorded initially. In endolymph, the endocochlear potential decreased by 5 mV/h while its K+ concentration and osmolality increased by about 3 mM/h and 4 mosmol/kg H2O per hour, respectively. These results suggest that the negative surface charges demonstrated on Reissner's membrane may play a role in the homeostasis of endolymph. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png European Archives of Oto-Rhino-Laryngology Springer Journals

Changes in the electrochemical composition of cochlear fluids after intracisternal application of doxorubicin in the rat

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References (22)

Publisher
Springer Journals
Copyright
Copyright © 1992 by Springer-Verlag
Subject
Medicine & Public Health; Otorhinolaryngology; Neurosurgery; Head and Neck Surgery
ISSN
0937-4477
eISSN
1434-4726
DOI
10.1007/BF00183490
Publisher site
See Article on Publisher Site

Abstract

Since doxorubicin (Adriamycin) is known to bind on membranous negative surface charges, its effect on the electrochemical composition of the cochlear fluids was studied in rats. Doxorubicin was infused into the cerebrospinal fluid via the lateral cerebral ventricle. The endocochlear resting potential was recorded, and endolymph and perilymph of the scala vestibuli were collected from the basal cochlear turn before and 1, 2, and 4 h after the drug application. Na+, K+, and Cl− concentrations and osmolality of the endolymph and perilymph were measured in 1 nl aliquots. In perilymph, Cl− concentration increased 4 h after doxorubicin treatment to reach a concentration 8 mM higher than recorded initially. In endolymph, the endocochlear potential decreased by 5 mV/h while its K+ concentration and osmolality increased by about 3 mM/h and 4 mosmol/kg H2O per hour, respectively. These results suggest that the negative surface charges demonstrated on Reissner's membrane may play a role in the homeostasis of endolymph.

Journal

European Archives of Oto-Rhino-Laryngology – Springer Journals

Published: Jul 8, 2004

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