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Simultaneous Detection of Seven Drugs of Abuse by the TriageTM Panel for Drugs of Abuse

Simultaneous Detection of Seven Drugs of Abuse by the TriageTM Panel for Drugs of Abuse This novel, competitive immunoassay simultaneously detects seven drugs of abuse in urine. A urine sample is placed in contact with lyophilized reagents, the reaction mixture is allowed to come to equilibrium (10 min), and then the whole mixture is applied to a solid phase that contains various immobilized antibodies in discrete drug-class-specific zones. After a washing step, the operator visually examines each zone for the presence of a red bar. The method incorporates present threshold concentrations that are independent for each drug. In the absence of drug or in the presence of drug in quantities less than the threshold concentration, no colored bar is visible. Samples containing drug(s) at or above the threshold concentration cause a red bar to appear for the appropriate drug(s). Positive and negative procedural control zones are incorporated into each determination. The performance of the assay methodology matches that of instrumented immunoassay systems. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Clinical Chemistry Oxford University Press

Simultaneous Detection of Seven Drugs of Abuse by the TriageTM Panel for Drugs of Abuse

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References (11)

Publisher
Oxford University Press
Copyright
© 1992 The American Association of Clinical Chemists, Inc.
ISSN
0009-9147
eISSN
1530-8561
DOI
10.1093/clinchem/38.9.1678
Publisher site
See Article on Publisher Site

Abstract

This novel, competitive immunoassay simultaneously detects seven drugs of abuse in urine. A urine sample is placed in contact with lyophilized reagents, the reaction mixture is allowed to come to equilibrium (10 min), and then the whole mixture is applied to a solid phase that contains various immobilized antibodies in discrete drug-class-specific zones. After a washing step, the operator visually examines each zone for the presence of a red bar. The method incorporates present threshold concentrations that are independent for each drug. In the absence of drug or in the presence of drug in quantities less than the threshold concentration, no colored bar is visible. Samples containing drug(s) at or above the threshold concentration cause a red bar to appear for the appropriate drug(s). Positive and negative procedural control zones are incorporated into each determination. The performance of the assay methodology matches that of instrumented immunoassay systems.

Journal

Clinical Chemistry – Oxford University Press

Published: Sep 1, 1992

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