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- Publisher
- Wiley
- Copyright
- Copyright © 2000 Wiley Subscription Services, Inc., A Wiley Company
- ISSN
- 0902-4441
- eISSN
- 1600-0609
- DOI
- 10.1034/j.1600-0609.2000.065004258.x
- Publisher site
- See Article on Publisher Site
Abstract
Abstract: We have applied an automated real‐time quantitative PCR assay using a double‐labeled fluorogenic probe to detect t(9;22)‐positive cells in haematological malignancies. The results are expressed as the ratio of chimeric bcr‐abl transcripts on abl transcripts. Highly reproducible results were obtained for t(9;22)‐positive K562 RNA. Ten copies of bcr‐abl DNA from a recombinant KW‐3 plasmid and one positive cell in 104 can be detected. Thirty‐two patients with chronic myeloid leukaemia (CML), 25 with acute leukaemia, 12 with myelodysplastic syndromes and 7 with other myeloproliferative syndromes were tested. Follow‐up data were obtained in bcr‐abl positive cases. Results were compared with those of conventional nested RT‐PCR and cytogenetics. Real‐time quantitative RT‐PCR values correlated well with both these methods. However, in some cases the only means of detecting early relapse or blastic transformation was to examine the kinetics of real‐time quantitative RT‐PCR. Thus, real‐time quantitative RT‐PCR appears suitable for the diagnosis and follow‐up of patients with the t(9;22) translocation.
Journal
European Journal of Haematology – Wiley
Published: Oct 1, 2000