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Neurochemical organization of the hypothalamic projection to the spinal cord in the rat

Neurochemical organization of the hypothalamic projection to the spinal cord in the rat The hypothalamus provides a major projection to the spinal cord that innervates primarily lamina I of the dorsal horn and the sympathetic and parasympathetic preganglionic cell columns. We have examined the chemical organization of the neurons that contribute to this pathway by using combined retrograde transport of fluorescent dyes and immunohistochemistry for 15 different putative neurotransmitters or their synthetic enzymes. Our results demonstrate that 5 cytoarchitectonically distinct cell groups in the hypothalamus contribute to the spinal projection and that each has its own predominant chemical types. In the paraventricular nucleus, substantial numbers of hypothalamo‐spinal neurons stain with antisera against arginine vasopressin (25‐35%), oxytocin (20‐25%), and metenkephalin (10%). About 25% of the neurons with spinal projections in the retrochiasmatic area stain with an antiserum against α‐melanocyte‐stimulating hormone. Nearly 100% of the hypothalamo‐spinal neurons in the tuberal lateral hypothalamic area stain with this same antiserum, but these cells do not stain for other proopiomelanocortin‐derived peptides, and so probably contain a cross‐reacting peptide. This population must be distinguished from an adjacent cell group, in the perifornical region, where many spinal projection neurons stain with antisera against dynorphin (25%) or atrial natriuretic peptide (20%). Finally, in the dorsal hypothalamic area as many as 55‐75% of the neurons with spinal projections are dopaminergic, on the basis of their staining with an antiserum against tyrosine hydroxylase. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Comparative Neurology Wiley

Neurochemical organization of the hypothalamic projection to the spinal cord in the rat

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References (111)

Publisher
Wiley
Copyright
"Copyright © 1988 Wiley Subscription Services, Inc., A Wiley Company"
ISSN
0021-9967
eISSN
1096-9861
DOI
10.1002/cne.902720410
pmid
2901438
Publisher site
See Article on Publisher Site

Abstract

The hypothalamus provides a major projection to the spinal cord that innervates primarily lamina I of the dorsal horn and the sympathetic and parasympathetic preganglionic cell columns. We have examined the chemical organization of the neurons that contribute to this pathway by using combined retrograde transport of fluorescent dyes and immunohistochemistry for 15 different putative neurotransmitters or their synthetic enzymes. Our results demonstrate that 5 cytoarchitectonically distinct cell groups in the hypothalamus contribute to the spinal projection and that each has its own predominant chemical types. In the paraventricular nucleus, substantial numbers of hypothalamo‐spinal neurons stain with antisera against arginine vasopressin (25‐35%), oxytocin (20‐25%), and metenkephalin (10%). About 25% of the neurons with spinal projections in the retrochiasmatic area stain with an antiserum against α‐melanocyte‐stimulating hormone. Nearly 100% of the hypothalamo‐spinal neurons in the tuberal lateral hypothalamic area stain with this same antiserum, but these cells do not stain for other proopiomelanocortin‐derived peptides, and so probably contain a cross‐reacting peptide. This population must be distinguished from an adjacent cell group, in the perifornical region, where many spinal projection neurons stain with antisera against dynorphin (25%) or atrial natriuretic peptide (20%). Finally, in the dorsal hypothalamic area as many as 55‐75% of the neurons with spinal projections are dopaminergic, on the basis of their staining with an antiserum against tyrosine hydroxylase.

Journal

The Journal of Comparative Neurology – Wiley

Published: Oct 22, 1989

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