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Cytokinin biochemistry in relation to leaf senescence I. The metabolism of 6-benzylaminopurine and zeatin in oat leaf segments

Cytokinin biochemistry in relation to leaf senescence I. The metabolism of 6-benzylaminopurine... The metabolism of zeatin and that of 6-benzylaminopurine (BAP) have been compared in oat leaf segments in relation to the markedly differing ability of these cytokinins to retard senescence of such segments. Free BAP and a highly active senescence-retarding metabolite of BAP were detected in oat leaf segments supplied with BAP. The metabolite was identified by mass spectrometry and chromatography as 3-β-D-glucopyranosyl-BAP. The major metabolite of BAP was the 9-glucoside, but this lacked significant senescence-retarding activity. In contrast, in leaf segments supplied with zeatin, no free zeatin and no senescence-retarding metabolite of zeatin were detectable. The major metabolites of zeatin were adenosine, adenine nucleotides, the 9-glucoside, and unidentified polar metabolites. The differing activities of zeatin and BAP in the oat-leaf senescence bioassay appear to be, at least partially, a consequence of their differing metabolism and are not attributable to differences in uptake. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Plant Growth Regulation Springer Journals

Cytokinin biochemistry in relation to leaf senescence I. The metabolism of 6-benzylaminopurine and zeatin in oat leaf segments

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References (29)

Publisher
Springer Journals
Copyright
Copyright © Springer-Verlag 1983
ISSN
0721-7595
eISSN
1435-8107
DOI
10.1007/bf02042237
Publisher site
See Article on Publisher Site

Abstract

The metabolism of zeatin and that of 6-benzylaminopurine (BAP) have been compared in oat leaf segments in relation to the markedly differing ability of these cytokinins to retard senescence of such segments. Free BAP and a highly active senescence-retarding metabolite of BAP were detected in oat leaf segments supplied with BAP. The metabolite was identified by mass spectrometry and chromatography as 3-β-D-glucopyranosyl-BAP. The major metabolite of BAP was the 9-glucoside, but this lacked significant senescence-retarding activity. In contrast, in leaf segments supplied with zeatin, no free zeatin and no senescence-retarding metabolite of zeatin were detectable. The major metabolites of zeatin were adenosine, adenine nucleotides, the 9-glucoside, and unidentified polar metabolites. The differing activities of zeatin and BAP in the oat-leaf senescence bioassay appear to be, at least partially, a consequence of their differing metabolism and are not attributable to differences in uptake.

Journal

Journal of Plant Growth Regulation – Springer Journals

Published: Aug 1, 1983

Keywords: Leaf Senescence; Zeatin; Zeatin Riboside; Radish Cotyledon; Ethyl Methyl Ketone

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