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Birth of Normal Calves Resulting from Bovine Oocytes Matured, Fertilized, and Cultured with Cumulus Cells in Vitro up to the Blastocyst Stage

Birth of Normal Calves Resulting from Bovine Oocytes Matured, Fertilized, and Cultured with... Abstract Bovine oocytes matured in vitro were fertilized in high proportions (92% of matured oocytes) by sperm capacitated with Ca ionophore A23187. Eight percent of inseminated oocytes that were denuded 96 h after insemination developed to the morula stage when cultured for 6 – 120 h after insemination with cumulus cells from the original oocytes. Inseminated oocytes denuded 96 h after insemination developed to the blastocyst stage when cultured with or without cumulus cells or in the conditioned medium from 96 h to 168 – 216 h after insemination (9.0%, 8.1%, and 6.8% of inseminated oocytes respectively). Six frozen-thawed blastocysts were transferred nonsurgically to 3 recipients (2 embryos/recipient). Two of the 3 recipients became pregnant, with one delivering live twins at term. Seven fresh blastocysts were transferred nonsurgically to 6 recipients (1-2 embryos/recipient). Three of the 6 recipients became pregnant, with 2 delivering live calves. This content is only available as a PDF. Author notes 1 This work was supported by the Kitasato University, School of Veterinary Medicine and Animal Sciences research grant (Nos. 6012 and 6215). copyright © 1990 by The Society for the Study of Reproduction http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biology of Reproduction Oxford University Press

Birth of Normal Calves Resulting from Bovine Oocytes Matured, Fertilized, and Cultured with Cumulus Cells in Vitro up to the Blastocyst Stage

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Publisher
Oxford University Press
Copyright
copyright © 1990 by The Society for the Study of Reproduction
ISSN
0006-3363
eISSN
1529-7268
DOI
10.1095/biolreprod42.1.114
Publisher site
See Article on Publisher Site

Abstract

Abstract Bovine oocytes matured in vitro were fertilized in high proportions (92% of matured oocytes) by sperm capacitated with Ca ionophore A23187. Eight percent of inseminated oocytes that were denuded 96 h after insemination developed to the morula stage when cultured for 6 – 120 h after insemination with cumulus cells from the original oocytes. Inseminated oocytes denuded 96 h after insemination developed to the blastocyst stage when cultured with or without cumulus cells or in the conditioned medium from 96 h to 168 – 216 h after insemination (9.0%, 8.1%, and 6.8% of inseminated oocytes respectively). Six frozen-thawed blastocysts were transferred nonsurgically to 3 recipients (2 embryos/recipient). Two of the 3 recipients became pregnant, with one delivering live twins at term. Seven fresh blastocysts were transferred nonsurgically to 6 recipients (1-2 embryos/recipient). Three of the 6 recipients became pregnant, with 2 delivering live calves. This content is only available as a PDF. Author notes 1 This work was supported by the Kitasato University, School of Veterinary Medicine and Animal Sciences research grant (Nos. 6012 and 6215). copyright © 1990 by The Society for the Study of Reproduction

Journal

Biology of Reproduction – Oxford University Press

Published: Jan 1, 1990

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