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Simplified Radioimmunoassay for Serum Aldosterone Utilizing Increased Antibody Specificity

Simplified Radioimmunoassay for Serum Aldosterone Utilizing Increased Antibody Specificity ABSTRACT A shortened, simplified radioimmunoassay for serum aldosterone has been made possible by the development of a highly specific antiserum for aldosterone. This high specificity is largely due to the careful purification of the aldosterone-3-oxime derivative. Using 50% displacement of 1,2-3H-aldosterone as a measure, no other steroid cross-reacts to a degree greater than 0.015% (18-OH-DOC) and most cross-react less than 0.00003%. Recovery, accuracy and sensitivity are quite satisfactory using only serum extraction with methylene chloride and no further separation. The normal range found is comparable with others reported (3–35 ng/100 ml) although women are shown to have more variable levels than men. This content is only available as a PDF. Author notes Supported by the Canadian Medical Research Council Grant No. MA 4044. Copyright © 1974 by The Endocrine Society http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Clinical Endocrinology and Metabolism Oxford University Press

Simplified Radioimmunoassay for Serum Aldosterone Utilizing Increased Antibody Specificity

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Publisher
Oxford University Press
Copyright
Copyright © 1974 by The Endocrine Society
ISSN
0021-972X
eISSN
1945-7197
DOI
10.1210/jcem-38-4-622
Publisher site
See Article on Publisher Site

Abstract

ABSTRACT A shortened, simplified radioimmunoassay for serum aldosterone has been made possible by the development of a highly specific antiserum for aldosterone. This high specificity is largely due to the careful purification of the aldosterone-3-oxime derivative. Using 50% displacement of 1,2-3H-aldosterone as a measure, no other steroid cross-reacts to a degree greater than 0.015% (18-OH-DOC) and most cross-react less than 0.00003%. Recovery, accuracy and sensitivity are quite satisfactory using only serum extraction with methylene chloride and no further separation. The normal range found is comparable with others reported (3–35 ng/100 ml) although women are shown to have more variable levels than men. This content is only available as a PDF. Author notes Supported by the Canadian Medical Research Council Grant No. MA 4044. Copyright © 1974 by The Endocrine Society

Journal

Journal of Clinical Endocrinology and Metabolism – Oxford University Press

Published: Apr 1, 1974

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