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Rolling-circle amplification in DNA diagnostics: the power of simplicity

Rolling-circle amplification in DNA diagnostics: the power of simplicity Due to its robustness and simplicity, the rolling replication of circular DNA probes holds a distinct position in DNA diagnostics among other isothermal methods of target, probe or signal amplification. Major rolling-circle amplification approaches to DNA detection via posthybridization probe/signal turn-by-turn enhancement are briefly overviewed here with an emphasis on the new concepts and latest progress in the field, including the single-molecule and single-mutation detection assays as exemplary applications. Underlying mechanisms, current controversies and principal advantages of rolling-circle amplification are also considered. Possible future directions for the further advancement of this diagnostic methodology are outlined. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Expert Review of Molecular Diagnostics Taylor & Francis

Rolling-circle amplification in DNA diagnostics: the power of simplicity

Expert Review of Molecular Diagnostics , Volume 2 (6): 7 – Nov 1, 2002
7 pages

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References (60)

Publisher
Taylor & Francis
Copyright
© 2002 Future Drugs Ltd. All rights reserved
ISSN
1744-8352
eISSN
1473-7159
DOI
10.1586/14737159.2.6.542
pmid
12465451
Publisher site
See Article on Publisher Site

Abstract

Due to its robustness and simplicity, the rolling replication of circular DNA probes holds a distinct position in DNA diagnostics among other isothermal methods of target, probe or signal amplification. Major rolling-circle amplification approaches to DNA detection via posthybridization probe/signal turn-by-turn enhancement are briefly overviewed here with an emphasis on the new concepts and latest progress in the field, including the single-molecule and single-mutation detection assays as exemplary applications. Underlying mechanisms, current controversies and principal advantages of rolling-circle amplification are also considered. Possible future directions for the further advancement of this diagnostic methodology are outlined.

Journal

Expert Review of Molecular DiagnosticsTaylor & Francis

Published: Nov 1, 2002

Keywords: circular probes; DNA diagnostics; PNA openers; strand displacement; rolling-circle amplification; topological labeling; single nucleotide polymorphisms

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