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Due to its robustness and simplicity, the rolling replication of circular DNA probes holds a distinct position in DNA diagnostics among other isothermal methods of target, probe or signal amplification. Major rolling-circle amplification approaches to DNA detection via posthybridization probe/signal turn-by-turn enhancement are briefly overviewed here with an emphasis on the new concepts and latest progress in the field, including the single-molecule and single-mutation detection assays as exemplary applications. Underlying mechanisms, current controversies and principal advantages of rolling-circle amplification are also considered. Possible future directions for the further advancement of this diagnostic methodology are outlined.
Expert Review of Molecular Diagnostics – Taylor & Francis
Published: Nov 1, 2002
Keywords: circular probes; DNA diagnostics; PNA openers; strand displacement; rolling-circle amplification; topological labeling; single nucleotide polymorphisms
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