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Indirect evidence for cholinergic inhibition of intestinal bicarbonate absorption in humans

Indirect evidence for cholinergic inhibition of intestinal bicarbonate absorption in humans BACKGROUND The aim of the study was to test the hypothesis that in the fasting state, proximal intestinal HCO3 − absorption, which depends on villus Na+/H+ exchanger activity, is tonically inhibited by a cholinergic atropine sensitive mechanism. SUBJECTS The experiments were performed in 34 healthy volunteers and in eight patients with intestinal villus atrophy. METHODS HCO3 −absorption was measured with a modified triple lumen perfusion technique in the distal duodenum, the most proximal portion of the small intestine. The study was designed to compensate for the inhibitory effects of atropine on intestinal motor activity. RESULTS Atropine had three effects on HCO3 − transport: it reduced HCO3 − concentration at the proximal aspiration site, it displaced the relation between HCO3 − concentration and HCO3 − absorption to the left, and it induced a significant acidification of the perfusate at the distal aspiration site. The magnitude of the stimulatory effect on HCO3 − absorption was similar to the difference between patients with intestinal villus atrophy and healthy controls. CONCLUSION The data suggest that, in the fasting state, duodenal HCO3 − absorption, which depends on villus Na+/H+ exchanger activity, may be tonically inhibited by an atropine sensitive cholinergic mechanism. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Gut British Medical Journal

Indirect evidence for cholinergic inhibition of intestinal bicarbonate absorption in humans

Mellander, A; Sjövall, H
Gut , Volume 44 (3) – Mar 1, 1999
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Indirect evidence for cholinergic inhibition of intestinal bicarbonate absorption in humans

Mellander, A; Sjövall, H
Gut , Volume 44 (3) – Mar 1, 1999

Abstract



BACKGROUND
The aim of the study was to test the hypothesis that in the fasting state, proximal intestinal HCO3
− absorption, which depends on villus Na+/H+ exchanger activity, is tonically inhibited by a cholinergic atropine sensitive mechanism.


SUBJECTS
The experiments were performed in 34 healthy volunteers and in eight patients with intestinal villus atrophy.


METHODS
HCO3
−absorption was measured with a modified triple lumen perfusion technique in the distal duodenum, the most proximal portion of the small intestine. The study was designed to compensate for the inhibitory effects of atropine on intestinal motor activity.


RESULTS
Atropine had three effects on HCO3
− transport: it reduced HCO3
− concentration at the proximal aspiration site, it displaced the relation between HCO3
− concentration and HCO3
− absorption to the left, and it induced a significant acidification of the perfusate at the distal aspiration site. The magnitude of the stimulatory effect on HCO3
− absorption was similar to the difference between patients with intestinal villus atrophy and healthy controls.


CONCLUSION
The data suggest that, in the fasting state, duodenal HCO3
− absorption, which depends on villus Na+/H+ exchanger activity, may be tonically inhibited by an atropine sensitive cholinergic mechanism.

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Publisher
British Medical Journal
Copyright
British Society of Gastroenterology
ISSN
0017-5749
eISSN
1468-3288
DOI
10.1136/gut.44.3.353
Publisher site
See Article on Publisher Site

Abstract

BACKGROUND The aim of the study was to test the hypothesis that in the fasting state, proximal intestinal HCO3 − absorption, which depends on villus Na+/H+ exchanger activity, is tonically inhibited by a cholinergic atropine sensitive mechanism. SUBJECTS The experiments were performed in 34 healthy volunteers and in eight patients with intestinal villus atrophy. METHODS HCO3 −absorption was measured with a modified triple lumen perfusion technique in the distal duodenum, the most proximal portion of the small intestine. The study was designed to compensate for the inhibitory effects of atropine on intestinal motor activity. RESULTS Atropine had three effects on HCO3 − transport: it reduced HCO3 − concentration at the proximal aspiration site, it displaced the relation between HCO3 − concentration and HCO3 − absorption to the left, and it induced a significant acidification of the perfusate at the distal aspiration site. The magnitude of the stimulatory effect on HCO3 − absorption was similar to the difference between patients with intestinal villus atrophy and healthy controls. CONCLUSION The data suggest that, in the fasting state, duodenal HCO3 − absorption, which depends on villus Na+/H+ exchanger activity, may be tonically inhibited by an atropine sensitive cholinergic mechanism.

Journal

Gut – British Medical Journal

Published: Mar 1, 1999

Keywords: small intestine absorption cholinergic muscarinic receptor villus atrophy coeliac disease

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