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Analytic Techniques in the Separation and Identification of Specific Purine and Pyrimidine Degradation Products of tRNA: Application to Urine Samples From Cancer Patients

Analytic Techniques in the Separation and Identification of Specific Purine and Pyrimidine... Summary High-resolution liquid chromatographic procedures for the separation and analysis of several nucleosides and purine bases were applied to the separation of trace urinary degradation products of tRNA in humans and their subsequent identification by means of gas chromatography (GC), mass spectrometry (MS), and combined GC-MS. Purine and pyrimidine products present in all urine samples included pseudouridine (ψrd), 1-methylinosine (m1l), N2,N2-dimethylguanosine (m22G), 1-methylguanosine (m1G), and 7-methylguanine (7-MeGua). The excretion of these compounds in the urine of normal control adults were, in mg/kg/24 hours: ψrd, 0.94; m1l, 0.06; m22, 0.06; m1G, 0.07; and 7-MeGua, 0.10. Clinically normal children from 4 to 13 years old excreted about 60% more of these compounds than did the adult controls. Adult patients with neoplastic diseases, including lung cancer, leukemia, Burkitt's lymphoma, melanoma, and ovarian cancer, showed elevated urinary excretion of ψrd, m1l, and m1l, 0.06; m22. Results of a few analyses for m1G and 7-MeGua generally seemed to parallel those of the other methylated compounds. 2 Supported by Public Health Service contract NIH-CA-71-59 from the National Cancer Institute. This content is only available as a PDF. Author notes 3 Oak Ridge National Laboratory, Oak Ridge, Tenn. 37830. Operated for the U.S. Atomic Energy Commission by Union Carbide Corp. 4 Laboratory of Chemical Pharmacology, National Cancer Institute, National Institutes of Health, Public Health Service, U.S. Department of Health, Education, and Welfare, Bethesda, Md. 20014. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png JNCI: Journal of the National Cancer Institute Oxford University Press

Analytic Techniques in the Separation and Identification of Specific Purine and Pyrimidine Degradation Products of tRNA: Application to Urine Samples From Cancer Patients

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Publisher
Oxford University Press
ISSN
0027-8874
eISSN
1460-2105
DOI
10.1093/jnci/53.6.1553
Publisher site
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Abstract

Summary High-resolution liquid chromatographic procedures for the separation and analysis of several nucleosides and purine bases were applied to the separation of trace urinary degradation products of tRNA in humans and their subsequent identification by means of gas chromatography (GC), mass spectrometry (MS), and combined GC-MS. Purine and pyrimidine products present in all urine samples included pseudouridine (ψrd), 1-methylinosine (m1l), N2,N2-dimethylguanosine (m22G), 1-methylguanosine (m1G), and 7-methylguanine (7-MeGua). The excretion of these compounds in the urine of normal control adults were, in mg/kg/24 hours: ψrd, 0.94; m1l, 0.06; m22, 0.06; m1G, 0.07; and 7-MeGua, 0.10. Clinically normal children from 4 to 13 years old excreted about 60% more of these compounds than did the adult controls. Adult patients with neoplastic diseases, including lung cancer, leukemia, Burkitt's lymphoma, melanoma, and ovarian cancer, showed elevated urinary excretion of ψrd, m1l, and m1l, 0.06; m22. Results of a few analyses for m1G and 7-MeGua generally seemed to parallel those of the other methylated compounds. 2 Supported by Public Health Service contract NIH-CA-71-59 from the National Cancer Institute. This content is only available as a PDF. Author notes 3 Oak Ridge National Laboratory, Oak Ridge, Tenn. 37830. Operated for the U.S. Atomic Energy Commission by Union Carbide Corp. 4 Laboratory of Chemical Pharmacology, National Cancer Institute, National Institutes of Health, Public Health Service, U.S. Department of Health, Education, and Welfare, Bethesda, Md. 20014.

Journal

JNCI: Journal of the National Cancer Institute – Oxford University Press

Published: Dec 1, 1974

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