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References (10)
-
G. Baird, D. Zacharias, R. Tsien (2000)
Biochemistry, mutagenesis, and oligomerization of DsRed, a red fluorescent protein from coral.Proceedings of the National Academy of Sciences of the United States of America, 97 22
-
R. Campbell, Oded Tour, A. Palmer, Paul Steinbach, G. Baird, D. Zacharias, R. Tsien (2002)
A monomeric red fluorescent proteinProceedings of the National Academy of Sciences of the United States of America, 99
-
N. Dunham, T. Miya (1957)
A note on a simple apparatus for detecting neurological deficit in rats and mice.Journal of the American Pharmaceutical Association. American Pharmaceutical Association, 46 3
-
Meng Yang, P. Jiang, F. Sun, S. Hasegawa, E. Baranov, T. Chishima, H. Shimada, A. Moossa, R. Hoffman (1999)
A fluorescent orthotopic bone metastasis model of human prostate cancer.Cancer research, 59 4
-
Jia Zeng, Shanfeng Zhu, Alan Liew, Hong Yan (2010)
Multiconstrained gene clustering based on generalized projectionsBMC Bioinformatics, 11
-
S. Srinivas, Tomoko Watanabe, Chyuan-Sheng Lin, C. William, Y. Tanabe, T. Jessell, F. Costantini (2001)
Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locusBMC Developmental Biology, 1
-
M. Matz, A. Fradkov, Y. Labas, A. Savitsky, A. Zaraisky, M. Markelov, S. Lukyanov (1999)
Fluorescent proteins from nonbioluminescent Anthozoa speciesNature Biotechnology, 17
-
M. Okabe, M. Ikawa, K. Kominami, T. Nakanishi, Y. Nishimune (1997)
‘Green mice’ as a source of ubiquitous green cellsFEBS Letters, 407
-
B. Zambrowicz, A. Imamoto, S. Fiering, L. Herzenberg, W. Kerr, Philippe Soriano (1997)
Disruption of overlapping transcripts in the ROSA beta geo 26 gene trap strain leads to widespread expression of beta-galactosidase in mouse embryos and hematopoietic cells.Proceedings of the National Academy of Sciences of the United States of America, 94 8
-
AK Hadjantonakis, S Macmaster, A Nagy (2002)
Embryonic stem cells and mice expressing different GFP variants for multiple non‐invasive reporter usage within a single animalBMC Biotechnol, 2
- Publisher
- Wiley
- Copyright
- Copyright © 2005 Wiley Subscription Services, Inc., A Wiley Company
- ISSN
- 1526-954X
- eISSN
- 1526-968X
- DOI
- 10.1002/gene.20129
- pmid
- 15880439
- Publisher site
- See Article on Publisher Site
Abstract
Fluorescent proteins provide a powerful means to track gene expression and cellular behaviors in the study of model organisms such as mice. Among the new generation of fluorescent protein markers, the monomeric red fluorescent protein mRFP1 is particularly attractive because of its rapid maturation and minimal interference with GFP and GFP‐derived markers. Here we evaluate the utility of mRFP1 as a marker in transgenic mice. We show that high level and ubiquitous expression of mRFP1 does not affect mouse development, general physiology, or reproduction. mRFP1 expression can be readily detected with unaided eyes under daylight in transgenic mice on the albino background. The intensity of mRFP1 signals can be used to distinguish homozygous and heterozygous transgenic mice. Together, these features make mRFP1 an attractive marker for broad applications in transgenic research. genesis 42:86–90, 2005. © 2005 Wiley‐Liss, Inc.
Journal
Genesis: the Journal of Genetics and Development – Wiley
Published: Jun 1, 2005
Keywords: ; ; ; ;