Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 7-Day Trial for You or Your Team.

Learn More →

Arabidopsis knockout mutation of ADC2 gene reveals inducibility by osmotic stress

Arabidopsis knockout mutation of ADC2 gene reveals inducibility by osmotic stress We isolated an Arabidopsis thaliana mutant line carrying an insertion of the En‐1 transposable element at the ADC2 locus. The insertion causes a knockout of the arginine decarboxylase 2 gene. We demonstrated that ADC2 is the gene responsible for induction of the polyamine biosynthetic pathway by osmotic stress. No induction of ADC activity by the osmolite sorbitol could be observed in the homozygous mutant, indicating a predominant role of ADC2 in stress response. ADC activity is reduced in the mutant by 44% under non‐stressed conditions and the mutant shows no obvious phenotype. This is the first report of a genetically mapped mutation in the polyamine biosynthetic pathway in plants. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png FEBS Letters Wiley

Arabidopsis knockout mutation of ADC2 gene reveals inducibility by osmotic stress

Soyka, Stephan; Heyer, Arnd G.
FEBS Letters , Volume 458 (2) – Sep 17, 1999
Download PDF
5 pages

Loading next page...
 
/lp/wiley/arabidopsis-knockout-mutation-of-adc2-gene-reveals-inducibility-by-pEXd2JfZAS

References (41)

Publisher
Wiley
Copyright
© 2015 Federation of European Biochemical Societies
eISSN
1873-3468
DOI
10.1016/S0014-5793(99)01125-4
Publisher site
See Article on Publisher Site

Abstract

We isolated an Arabidopsis thaliana mutant line carrying an insertion of the En‐1 transposable element at the ADC2 locus. The insertion causes a knockout of the arginine decarboxylase 2 gene. We demonstrated that ADC2 is the gene responsible for induction of the polyamine biosynthetic pathway by osmotic stress. No induction of ADC activity by the osmolite sorbitol could be observed in the homozygous mutant, indicating a predominant role of ADC2 in stress response. ADC activity is reduced in the mutant by 44% under non‐stressed conditions and the mutant shows no obvious phenotype. This is the first report of a genetically mapped mutation in the polyamine biosynthetic pathway in plants.

Journal

FEBS Letters – Wiley

Published: Sep 17, 1999

Keywords: ; ; ;

There are no references for this article.