Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 7-Day Trial for You or Your Team.

Learn More →

Interaction of melittin with a human lymphoblastoid cell line, HMy2

Interaction of melittin with a human lymphoblastoid cell line, HMy2 We have examined the cytolytic effects of the membrane‐active peptide, melittin, on a human lymphoblastoid cell line (HMy2) in the context of the use of melittin as the toxic component of an immunotoxin. The toxicity of melittin for HMy2 cells was linear over the concentration range 0.875–3.5 ॖM. Increased incubation times failed to result in significant cell death at concentrations of melittin below 0.875 ॖM. Kinetic analysis revealed that the cytolytic activity of melittin was independent of time of exposure beyond 90 min. Flow cytometric analysis of HMy2 cells incubated with FITC‐labeled melittin demonstrated that the cells could incorporate up to 2.5 × 105 FITC‐melittin molecules per cell with no reduction in viability. Extrapolation of this data indicates that 106 melittin molecules per cell are required for maximum cytotoxicity to HMy2 cells. Further analysis of HMy2 cells that incorporated melittin, but that remained viable, revealed that these cells were able to reduce the number of melittin molecules per cell over time. The data indicate a potential threshold value for the number of melittin molecules that may be required to be delivered to the cell surface in the form of an immunotoxin if effective selective cell death is to be achieved. J. Cell. Biochem. 68:164–173, 1998. © 1998 Wiley‐Liss, Inc. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Cellular Biochemistry Wiley

Interaction of melittin with a human lymphoblastoid cell line, HMy2

Download PDF
10 pages

Loading next page...
 
/lp/wiley/interaction-of-melittin-with-a-human-lymphoblastoid-cell-line-hmy2-oMMJkh8YBC

References (0)

References for this paper are not available at this time. We will be adding them shortly, thank you for your patience.

Publisher
Wiley
Copyright
Copyright © 1998 Wiley‐Liss, Inc.
ISSN
0730-2312
eISSN
1097-4644
DOI
10.1002/(SICI)1097-4644(19980201)68:2<164::AID-JCB3>3.3.CO;2-I
Publisher site
See Article on Publisher Site

Abstract

We have examined the cytolytic effects of the membrane‐active peptide, melittin, on a human lymphoblastoid cell line (HMy2) in the context of the use of melittin as the toxic component of an immunotoxin. The toxicity of melittin for HMy2 cells was linear over the concentration range 0.875–3.5 ॖM. Increased incubation times failed to result in significant cell death at concentrations of melittin below 0.875 ॖM. Kinetic analysis revealed that the cytolytic activity of melittin was independent of time of exposure beyond 90 min. Flow cytometric analysis of HMy2 cells incubated with FITC‐labeled melittin demonstrated that the cells could incorporate up to 2.5 × 105 FITC‐melittin molecules per cell with no reduction in viability. Extrapolation of this data indicates that 106 melittin molecules per cell are required for maximum cytotoxicity to HMy2 cells. Further analysis of HMy2 cells that incorporated melittin, but that remained viable, revealed that these cells were able to reduce the number of melittin molecules per cell over time. The data indicate a potential threshold value for the number of melittin molecules that may be required to be delivered to the cell surface in the form of an immunotoxin if effective selective cell death is to be achieved. J. Cell. Biochem. 68:164–173, 1998. © 1998 Wiley‐Liss, Inc.

Journal

Journal of Cellular Biochemistry – Wiley

Published: Feb 1, 1998

Keywords: melittin; flow cytometry; cytotoxicity; immunotoxin; HMy2

There are no references for this article.