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Lack of specific inhibition of graft-versus-host reaction by anti-fab serum

Lack of specific inhibition of graft-versus-host reaction by anti-fab serum 190 Speeialia EXPERIENTIA 26/2 Anti-immunoglobulin sera (algS) have been shown to Graft-versus-host reaction assay. CBA mouse lymph produce some effects on the immunological reactivity of node or spleen cells were incubated in medium 199 with lymphocytes. Anti-allotype sera transform lympho- various concentrations of RaMFabS and 10% calf serum cytes~; high titre aIgS inhibits the adoptive secondary at 37~ for 30 min; they were then washed and in some response 2. In the action of these sera, the possibility of a cases reincubated for 30 min with fresh rabbit serum reaction with a receptor arises 9 Therefore it is worth in- absorbed with mouse red cells as a source of complement quiring if they would act on a primary response, for in- (RC'); they were then again washed and injected in stance on the graft-versus-host reaction (GVHR); an various numbers into new-born F 1 (CBA � Balb/c) mice. experiment of this nature may also elucidate the role of The spleens of the recipients were weighed 10 days later. immunoglobulins in cellular immunity 9 Cytoxicity test. The technique of SANDERSON a modified Methods. Sera. The following sera were employed: by RUSZKIEWICZ 4, based on release of 51Cr from CBA rabbit anti-mouse http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Cellular and Molecular Life Sciences Springer Journals

Lack of specific inhibition of graft-versus-host reaction by anti-fab serum

Sternberg, M.
Cellular and Molecular Life Sciences , Volume 26 (2) – Feb 1, 1970
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Publisher
Springer Journals
Copyright
Copyright © Birkhäuser-Verlag 1970
ISSN
1420-682X
DOI
10.1007/bf01895575
Publisher site
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Abstract

190 Speeialia EXPERIENTIA 26/2 Anti-immunoglobulin sera (algS) have been shown to Graft-versus-host reaction assay. CBA mouse lymph produce some effects on the immunological reactivity of node or spleen cells were incubated in medium 199 with lymphocytes. Anti-allotype sera transform lympho- various concentrations of RaMFabS and 10% calf serum cytes~; high titre aIgS inhibits the adoptive secondary at 37~ for 30 min; they were then washed and in some response 2. In the action of these sera, the possibility of a cases reincubated for 30 min with fresh rabbit serum reaction with a receptor arises 9 Therefore it is worth in- absorbed with mouse red cells as a source of complement quiring if they would act on a primary response, for in- (RC'); they were then again washed and injected in stance on the graft-versus-host reaction (GVHR); an various numbers into new-born F 1 (CBA � Balb/c) mice. experiment of this nature may also elucidate the role of The spleens of the recipients were weighed 10 days later. immunoglobulins in cellular immunity 9 Cytoxicity test. The technique of SANDERSON a modified Methods. Sera. The following sera were employed: by RUSZKIEWICZ 4, based on release of 51Cr from CBA rabbit anti-mouse

Journal

Cellular and Molecular Life Sciences – Springer Journals

Published: Feb 1, 1970

Keywords: Specific Inhibition

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