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References (20)
-
Robert Winchester, S. Fu (1976)
Lymphocyte Surface Membrane ImmunoglobulinScandinavian Journal of Immunology, 5
-
H. Clark, C. Shepard (1963)
A DIALYSIS TECHNIQUE FOR PREPARING FLUORESCENT ANTIBODY.Virology, 20
-
T. Hallberg, B. Gurner, R. Coombs (1973)
Opsonic adherence of sensitized ox red cells to human lymphocytes as measured by rosette formation.International archives of allergy and applied immunology, 44 4
-
E. Vitetta, J. Uhr (1975)
Immunoglobulin-receptors revisited.Science, 189 4207
-
H. Dickler (1976)
Lymphocyte Binding of Aggregated ImmunoglobulinScandinavian Journal of Immunology, 5
-
M. Song, N. Adham, H. Rinderknecht (1975)
Large scale purification of α2-macroglobulin from human plasmaBiochemical Medicine, 14
-
M. Burstein, J. Samaille (1958)
Sur une nouvelle méthode de dosage du cholestérol. lié aux α et aux β-lipoprotéines du sérumClinica Chimica Acta, 3
-
G. Holm, D. Pettersson, H. Mellstedt, E. Hedfors, B. Bloth (1975)
Lymphocyte subpopulations in peripheral blood of healthy persons. Characterization by surface markers and lack of selection during purificationClinical and Experimental Immunology, 20
-
P. Lobo, F. Westervelt, D. Horwitz (1975)
Identification of two populations of immunoglobulin-bearing lymphocytes in man.Journal of immunology, 114 1 Pt 1
-
A. Nisonoff, F. Wissler, L. Lipman (1960)
Properties of the Major Component of a Peptic Digest of Rabbit AntibodyScience, 132
-
D. Mason, S. Labaume, J. Preud'homme (1977)
The detection of membrane and cytoplasmic immunoglobulins in human leucocytes by immunoperoxidase staining.Clinical and experimental immunology, 29 3
-
E. Engvall, P. Perlmann (1972)
Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes.Journal of immunology, 109 1
-
(1958)
A new method for the determination of cholesterol linked to serum x - lipoproteins and ^ - lipoproteins -
K. Kumagai, T. Abo, T. Sekizawa, M. Sasaki (1975)
Studies of surface immunoglobulins on human B lymphocytes. I. Dissociation of cell-bound immunoglobulins with acid pH or at 37 degrees C.Journal of immunology, 115 4
-
J. Porath, R. Axén, S. Ernback (1967)
Chemical Coupling of Proteins to AgaroseNature, 215
-
S. Avrameas, T. Ternynck (1969)
The cross-linking of proteins with glutaraldehyde and its use for the preparation of immunoadsorbents.Immunochemistry, 6 1
-
D. Horwitz, P. Lobo (1975)
Characterizaiton of two populations of human lymphocytes bearing easily detectable surface immunoglobulin.The Journal of clinical investigation, 56 6
-
Robert Winchester, S. Fu, Thomas Hoffman, Henry Kunkel (1975)
IgG on lymphocyte surfaces; technical problems and the significance of a third cell population.Journal of immunology, 114 4
-
(1976)
Surface markers ofhuman lymphocytes -
H. Hjelm, K. Hjelm, J. Sjöquist (1972)
Protein a from Staphylococcus aureus. Its isolation by affinity chromatography and its use as an immunosorbent for isolation of immunoglobulinsFEBS Letters, 28
- Publisher
- Wiley
- Copyright
- Copyright © 1978 Wiley Subscription Services, Inc., A Wiley Company
- ISSN
- 0300-9475
- eISSN
- 1365-3083
- DOI
- 10.1111/j.1365-3083.1978.tb00553.x
- Publisher site
- See Article on Publisher Site
Abstract
Surface immunoglobulins (sIg) on human blood lymphocytes were identified by immunofluorescence (IFL) after staining with conjugated F(ab'2) fragments of the anti‐Ig antibodies. A large fraction (≃20%) of freshly isolated lymphocytes was found to carry sIgG. Polyclonal IgG, which was present on Fc‐receptor‐bearing lymphocytes, could be removed by incubation and repeated washings only at 37°C. Lymphocytes treated at 37°C expressed the same percentage of sIg+ cells in direct IFL when F(ab')2 fragments of the antibody was used as when undigested aggregate‐free IgG antibody was used. Indirect IFL using F(ab')2 fragments in both steps yielded similar sIg+ values. However, much higher percentage of cells carried sIg when undigested antibody was included in one of the steps. The results suggest that incubation and washing at 37°C and the use of F(ab)2 fragments of the anybodies are important to eliminate absorbed sIgG and to avoid absorption of IgG during the staining procedure, thus preventing overestimation of the number of sIg+ B lymphocytes identified by IFL.
Journal
Scandinavian Journal of Immunology – Wiley
Published: Dec 1, 1978