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Insulin and Cortisol Promote Leptin Production in Cultured Human Fat Cells

Insulin and Cortisol Promote Leptin Production in Cultured Human Fat Cells Insulin and Cortisol Promote Leptin Production in Cultured Human Fat Cells Martin Wabitsch, Per Bo Jensen, Werner F. Blum, Claus T. Christoffersen, Piera Englaro, Eberhard Heinze, Wolfgang Rascher, Walter Teller, Hans Tornqvist, and Hans Hauner production in adipose tissue using the model of in vitro The aim of this study was to investigate the regulation differentiated human adipocytes. of leptin expression and production in cultured human adipocytes using the model of in vitro differentiated human adipocytes. Freshly isolated human preadipo- RESEARCH DESIGN AND METHODS cytes did not exhibit significant leptin mRNA and pro- Subjects. Human adipose tissue samples (wet weight 200-500 g) were tein levels as assessed by reverse transcriptase (RT)- obtained from the mammary adipose tissue depot of 10 healthy women (mean polymerase chain reaction (PCR) and radioimmunoas- age 28 ± 7 years [means ± SD], mean BMI 28.3 ± 2.3 kg/m ) undergoing mam- say (RIA). However, during differentiation induced by a mary reduction. All patients gave their informed consent, and the study pro- tocol was approved by the ethical committee of the University of Ulm. defined adipogenic serum-free medium, cellular leptin Cell culture. After removal, the adipose tissue samples were stored in phos- mRNA and leptin http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Diabetes Unpaywall

Insulin and Cortisol Promote Leptin Production in Cultured Human Fat Cells

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Unpaywall
ISSN
0012-1797
DOI
10.2337/diab.45.10.1435
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Abstract

Insulin and Cortisol Promote Leptin Production in Cultured Human Fat Cells Martin Wabitsch, Per Bo Jensen, Werner F. Blum, Claus T. Christoffersen, Piera Englaro, Eberhard Heinze, Wolfgang Rascher, Walter Teller, Hans Tornqvist, and Hans Hauner production in adipose tissue using the model of in vitro The aim of this study was to investigate the regulation differentiated human adipocytes. of leptin expression and production in cultured human adipocytes using the model of in vitro differentiated human adipocytes. Freshly isolated human preadipo- RESEARCH DESIGN AND METHODS cytes did not exhibit significant leptin mRNA and pro- Subjects. Human adipose tissue samples (wet weight 200-500 g) were tein levels as assessed by reverse transcriptase (RT)- obtained from the mammary adipose tissue depot of 10 healthy women (mean polymerase chain reaction (PCR) and radioimmunoas- age 28 ± 7 years [means ± SD], mean BMI 28.3 ± 2.3 kg/m ) undergoing mam- say (RIA). However, during differentiation induced by a mary reduction. All patients gave their informed consent, and the study pro- tocol was approved by the ethical committee of the University of Ulm. defined adipogenic serum-free medium, cellular leptin Cell culture. After removal, the adipose tissue samples were stored in phos- mRNA and leptin

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Diabetes – Unpaywall

Published: Oct 1, 1996

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