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Incorporation of Mevalonic Acid into Ribosylzeatin in Tobacco Callus Ribonucleic Acid Preparations

Incorporation of Mevalonic Acid into Ribosylzeatin in Tobacco Callus Ribonucleic Acid Preparations Abstract The incorporation of 14C-2-mevalonic acid into transfer RNA and ribosomal RNA (high molecular weight RNA) in rapidly growing, cytokinin-dependent tobacco (Nicotiana tabacum var. Wisconsin No. 38) callus cultures has been investigated. Approximately 40% of the label incorporated into transfer RNA was present in a ribonucleoside with chromatographic properties identical to those of cis-ribosylzeatin. The remainder of the label in the transfer RNA appears to be nonspecific incorporation resulting from degradation and metabolism of 14C-2-mevalonic acid by the tobacco callus tissue. Although the total radioactivity incorporated into ribosomal RNA was roughly the same as in transfer RNA, the specific radioactivity of the transfer RNA was about four times higher than that of the ribosomal RNA, and the ribosomal RNA labeling could be distinguished from the cytokinin labeling observed in transfer RNA. The distributions of the 14C-2-mevalonic acid label and cytokinin activity in tobacco callus transfer RNA fractionated by benzoylated diethylaminoethylcellulose chromatography indicate that at least two cytokinin-containing transfer RNA species are present in this tissue. 2 Post Doctoral Fellow supported in part by a research grant from E. I. Du Pont de Nemours and Co. Committee on Educational Aid to F. S. 3 Present address: Department of Botany and Plant Pathology, Oregon State University, Corvallis, Ore. 97331. 1 This work was supported in part by National Science Foundation Research Grant GB-35260X1 (BMS72-02226) to F. S. This content is only available as a PDF. © 1975 American Society of Plant Biologists This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model) http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Physiology Oxford University Press

Incorporation of Mevalonic Acid into Ribosylzeatin in Tobacco Callus Ribonucleic Acid Preparations

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References (30)

Publisher
Oxford University Press
Copyright
Copyright © 2021 American Society of Plant Biologists
ISSN
0032-0889
eISSN
1532-2548
DOI
10.1104/pp.55.5.853
Publisher site
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Abstract

Abstract The incorporation of 14C-2-mevalonic acid into transfer RNA and ribosomal RNA (high molecular weight RNA) in rapidly growing, cytokinin-dependent tobacco (Nicotiana tabacum var. Wisconsin No. 38) callus cultures has been investigated. Approximately 40% of the label incorporated into transfer RNA was present in a ribonucleoside with chromatographic properties identical to those of cis-ribosylzeatin. The remainder of the label in the transfer RNA appears to be nonspecific incorporation resulting from degradation and metabolism of 14C-2-mevalonic acid by the tobacco callus tissue. Although the total radioactivity incorporated into ribosomal RNA was roughly the same as in transfer RNA, the specific radioactivity of the transfer RNA was about four times higher than that of the ribosomal RNA, and the ribosomal RNA labeling could be distinguished from the cytokinin labeling observed in transfer RNA. The distributions of the 14C-2-mevalonic acid label and cytokinin activity in tobacco callus transfer RNA fractionated by benzoylated diethylaminoethylcellulose chromatography indicate that at least two cytokinin-containing transfer RNA species are present in this tissue. 2 Post Doctoral Fellow supported in part by a research grant from E. I. Du Pont de Nemours and Co. Committee on Educational Aid to F. S. 3 Present address: Department of Botany and Plant Pathology, Oregon State University, Corvallis, Ore. 97331. 1 This work was supported in part by National Science Foundation Research Grant GB-35260X1 (BMS72-02226) to F. S. This content is only available as a PDF. © 1975 American Society of Plant Biologists This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)

Journal

Plant Physiology – Oxford University Press

Published: May 1, 1975

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