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Cold-modulated expression of genes encoding for key enzymes of the sugar metabolism in spring and autumn cvs. of Beta vulgaris L.

Cold-modulated expression of genes encoding for key enzymes of the sugar metabolism in spring and... An integrated approach based on the use of bioinformatics and gene expression analysis tools was carried out to evaluate the organ-specific transcription modulation of nine genes relevant to sugar metabolism of Beta vulgaris L. plantlets of the autumn cv. Franca and spring cv. Bianca, in response to low-temperature (LT) treatments. Different growth cycles imply different plant capability to adapt to the environment that includes variations in gene expression of key metabolic enzymes. The transcriptional response was evaluated by quantitative PCR analysis before, during and after the LT treatments. The results were correlated with the LT-induced electrolyte leakage measure and the carbohydrate content. Stress-induced transcript level alterations were detected in the two cultivars, suggesting a modulation of sucrose synthesis and carbohydrate partitioning. Cold stress induced deep changes in the autumn cultivar, especially in fructose-1,6-biphosphatase gene expression, irrespective of temperature or exposure time. These differential features of expression profiles constitute first clues on the molecular basis of the differential LT response of sugarbeet autumn and spring cultivars. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Genetic Resources Cambridge University Press

Cold-modulated expression of genes encoding for key enzymes of the sugar metabolism in spring and autumn cvs. of Beta vulgaris L.

Pacifico, D.; Onofri, C.; Mandolino, G.
Plant Genetic Resources , Volume 9 (2): 4 – Jul 1, 2011
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References (11)

Publisher
Cambridge University Press
Copyright
Copyright © NIAB 2011
ISSN
1479-263X
eISSN
1479-2621
DOI
10.1017/S1479262111000438
Publisher site
See Article on Publisher Site

Abstract

An integrated approach based on the use of bioinformatics and gene expression analysis tools was carried out to evaluate the organ-specific transcription modulation of nine genes relevant to sugar metabolism of Beta vulgaris L. plantlets of the autumn cv. Franca and spring cv. Bianca, in response to low-temperature (LT) treatments. Different growth cycles imply different plant capability to adapt to the environment that includes variations in gene expression of key metabolic enzymes. The transcriptional response was evaluated by quantitative PCR analysis before, during and after the LT treatments. The results were correlated with the LT-induced electrolyte leakage measure and the carbohydrate content. Stress-induced transcript level alterations were detected in the two cultivars, suggesting a modulation of sucrose synthesis and carbohydrate partitioning. Cold stress induced deep changes in the autumn cultivar, especially in fructose-1,6-biphosphatase gene expression, irrespective of temperature or exposure time. These differential features of expression profiles constitute first clues on the molecular basis of the differential LT response of sugarbeet autumn and spring cultivars.

Journal

Plant Genetic Resources – Cambridge University Press

Published: Jul 1, 2011

Keywords: Beta vulgaris; cold stress; real-time PCR; sucrose metabolism; sugarbeet

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