Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 7-Day Trial for You or Your Team.

Learn More →

Biologically relevant metal ion‐dependent hydroxyl radical generation An update

Biologically relevant metal ion‐dependent hydroxyl radical generation An update Transition metal ions, especially iron, appear to be important mediators of oxidative damage in vivo. Iron(II) reacts with H2O2 to give more‐reactive radicals. On the basis of ESR spin‐trapping data with DMPO, supported by aromatic hydroxylation studies and patterns of DNA base modification, it is concluded that hydroxyl radical (OH•) is likely to be the major damaging species formed in Fenton Systems under biologically‐relevant conditions (which include iron concentrations no higher than the micromolar range). Although reactive oxo‐iron species (such as ferryl and perferryl) may also be important, chemical evidence for their formation and identity in biologically relevant Fenton systems is currently lacking. Studies at alkaline pH values show that iron(IV) and iron(V) species are highly oxidizing under those reaction conditions, with a pattern of reactivity different from that of OH•. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Febs Letters Wiley

Biologically relevant metal ion‐dependent hydroxyl radical generation An update

Halliwell, Barry; Gutteridge, John M.C.
Febs Letters , Volume 307 (1) – Jul 27, 1992
Download PDF
5 pages

Loading next page...
 
/lp/wiley/biologically-relevant-metal-ion-dependent-hydroxyl-radical-generation-ktCWylGFEP

References (62)

Publisher
Wiley
Copyright
© 2015 Federation of European Biochemical Societies
eISSN
1873-3468
DOI
10.1016/0014-5793(92)80911-Y
Publisher site
See Article on Publisher Site

Abstract

Transition metal ions, especially iron, appear to be important mediators of oxidative damage in vivo. Iron(II) reacts with H2O2 to give more‐reactive radicals. On the basis of ESR spin‐trapping data with DMPO, supported by aromatic hydroxylation studies and patterns of DNA base modification, it is concluded that hydroxyl radical (OH•) is likely to be the major damaging species formed in Fenton Systems under biologically‐relevant conditions (which include iron concentrations no higher than the micromolar range). Although reactive oxo‐iron species (such as ferryl and perferryl) may also be important, chemical evidence for their formation and identity in biologically relevant Fenton systems is currently lacking. Studies at alkaline pH values show that iron(IV) and iron(V) species are highly oxidizing under those reaction conditions, with a pattern of reactivity different from that of OH•.

Journal

Febs Letters – Wiley

Published: Jul 27, 1992

Keywords: ; ; ; ; ; ; ; ; ; ;

There are no references for this article.