Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 7-Day Trial for You or Your Team.

Learn More →

Scanning electron microscopic study of immunogold‐labeled human leukocytes

Scanning electron microscopic study of immunogold‐labeled human leukocytes For many years critical point drying (CPD) has been the method of choice for preparing cells for scanning electron microscopy (SEM). Described herein is a simple, efficient, inexpensive, reproducible, and safe procedure using Peldri II, a proprietary fluorocarbon compound that is solid at room temperature and a liquid above 25°C, as a sublimation dehydrant for processing specimens for SEM. The utility of Peldri II was demonstrated in studies using leukocytes from the blood of healthy donors and patients with leukemia as well as from long‐term lymphoblastoid cell lines. The application of the proposed Peldri II procedure was further documented in SEM studies in which the expression and distribution of the interleukin‐2 receptor (IL‐2R) on leukocyte surface membranes was imaged using colloidal gold‐labeled antibodies (i.e., immunogold). When compared with current SEM preparation procedures using CPD, Peldri II is a useful alternative that is thought to offer several important advantages. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Microscopy Research and Technique Wiley

Scanning electron microscopic study of immunogold‐labeled human leukocytes

Download PDF
9 pages

Loading next page...
 
/lp/wiley/scanning-electron-microscopic-study-of-immunogold-labeled-human-kkFrC4q2BN

References (23)

Publisher
Wiley
Copyright
Copyright © 1990 Wiley Subscription Services, Inc., A Wiley Company
ISSN
1059-910X
eISSN
1097-0029
DOI
10.1002/jemt.1060140403
pmid
2332804
Publisher site
See Article on Publisher Site

Abstract

For many years critical point drying (CPD) has been the method of choice for preparing cells for scanning electron microscopy (SEM). Described herein is a simple, efficient, inexpensive, reproducible, and safe procedure using Peldri II, a proprietary fluorocarbon compound that is solid at room temperature and a liquid above 25°C, as a sublimation dehydrant for processing specimens for SEM. The utility of Peldri II was demonstrated in studies using leukocytes from the blood of healthy donors and patients with leukemia as well as from long‐term lymphoblastoid cell lines. The application of the proposed Peldri II procedure was further documented in SEM studies in which the expression and distribution of the interleukin‐2 receptor (IL‐2R) on leukocyte surface membranes was imaged using colloidal gold‐labeled antibodies (i.e., immunogold). When compared with current SEM preparation procedures using CPD, Peldri II is a useful alternative that is thought to offer several important advantages.

Journal

Microscopy Research and Technique – Wiley

Published: Apr 1, 1990

Keywords: ; ; ; ; ; ;

There are no references for this article.