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Compartmentation and Equilibration of Abscisic Acid in Isolated Xanthium Cells

Compartmentation and Equilibration of Abscisic Acid in Isolated Xanthium Cells Abstract The compartmentation of endogenous abscisic acid (ABA), applied (±)-[3H]ABA, and (±)-trans-ABA was measured in isolated mesophyll cells of the Chicago strain of Xanthium strumarium L. The release of ABA to the medium in the presence or absence of DMSO was used to determine the equilibration of ABA in the cells. It was found that a greater percentage of the (±)-[3H]ABA and the (±)-trans-ABA was released into the medium than of the endogenous ABA, indicating that applied ABA did not equilibrate with the endogenous material. Therefore, in further investigations only the compartmentation of endogenous ABA was studied. Endogenous ABA was released from Xanthium cells according to the pH gradients among the various cellular compartments. Thus, darkness, high external pH, KNO2, and droughtstress all increased the efflux of ABA from the cells. Efflux of ABA from the cells in the presence of 0.6 m mannitol occurred within 30 seconds, but only 8% of the endogenous material was released during the 20 minute treatment. 2 Present address: Department of Botany and Plant Sciences, University of California, Riverside, CA 95251. 1 Supported by United States Department of Energy under Contract DE-AC02-76ER0-1338. This content is only available as a PDF. © 1986 American Society of Plant Biologists This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model) http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Physiology Oxford University Press

Compartmentation and Equilibration of Abscisic Acid in Isolated Xanthium Cells

Bray, Elizabeth A.; Zeevaart, Jan A. D.
Plant Physiology , Volume 80 (1) – Jan 1, 1986
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References (14)

Publisher
Oxford University Press
Copyright
Copyright © 2021 American Society of Plant Biologists
ISSN
0032-0889
eISSN
1532-2548
DOI
10.1104/pp.80.1.105
Publisher site
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Abstract

Abstract The compartmentation of endogenous abscisic acid (ABA), applied (±)-[3H]ABA, and (±)-trans-ABA was measured in isolated mesophyll cells of the Chicago strain of Xanthium strumarium L. The release of ABA to the medium in the presence or absence of DMSO was used to determine the equilibration of ABA in the cells. It was found that a greater percentage of the (±)-[3H]ABA and the (±)-trans-ABA was released into the medium than of the endogenous ABA, indicating that applied ABA did not equilibrate with the endogenous material. Therefore, in further investigations only the compartmentation of endogenous ABA was studied. Endogenous ABA was released from Xanthium cells according to the pH gradients among the various cellular compartments. Thus, darkness, high external pH, KNO2, and droughtstress all increased the efflux of ABA from the cells. Efflux of ABA from the cells in the presence of 0.6 m mannitol occurred within 30 seconds, but only 8% of the endogenous material was released during the 20 minute treatment. 2 Present address: Department of Botany and Plant Sciences, University of California, Riverside, CA 95251. 1 Supported by United States Department of Energy under Contract DE-AC02-76ER0-1338. This content is only available as a PDF. © 1986 American Society of Plant Biologists This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)

Journal

Plant Physiology – Oxford University Press

Published: Jan 1, 1986

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