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- Publisher
- Wiley
- Copyright
- Copyright © 1985 Wiley‐Liss, Inc.
- ISSN
- 0021-9541
- eISSN
- 1097-4652
- DOI
- 10.1002/jcp.1041220223
- pmid
- 3968189
- Publisher site
- See Article on Publisher Site
Abstract
10.1002/jcp.1041220223.abs Bovine pituitary fibroblast growth factor has been purified 222,000‐fold to homogeneity by a combination of differential salt extraction, gel filtration, and ion exchange chromatography on Mono S column. Pituitary FGF is a single‐chain polypeptide with an apparent molecular mass of 15,800 and an isoelectric point of 9.6. It is highly active in triggering the proliferation of bovine and human vascular endothelial cell (half‐maximal stimulation at 23–40 pg/ml (1.5–2.6 pM) and saturation between 140 and 280 pg/ml (9.3–18.6 pM)). It displays a similar activity on bovine vascular smooth muscle cells, corneal endothelial cells, granulosa and adrenal cortex cells, and rabbit costal chondrocytes.
Journal
Journal of Cellular Physiology – Wiley
Published: Feb 1, 1985