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Examination of the Gibberellins of Zea mays and Phaseolus multiflorus Using Thin-Layer Chromatography

Examination of the Gibberellins of Zea mays and Phaseolus multiflorus Using Thin-Layer... No. 4939 June 27, 1964 NATURE EXAMINATION OF THE GIBBERELLINS OF Zea mays AND Phaseolus multiflorus USING THIN-LAYER CHROMATOGRAPHY By D. F. JONES Imperial Chemical Industries, Ltd., Pharmaceutical Division, Akers Research Laboratories, Welwyn 1 2 REVIOUS publications • have described the identifica­ tied by their response to ethanolic sulphuric acid detection tion of microgramme amounts of the endogenous and by the characteristic migratory properties'·'·7 of gibberellins of some higher plant seeds by thin-layer the free acids and their derived methyl esters on thin­ chromatography. This technique has now been used to laye_r chromato~rap~y in the systems listed in the previous identify gibberellin A in an extract of Zea mays seed sectwn. Ident1ficatwns were made by direct comparison :<nd in quantitative analysis of the gibberellins in develop­ of RF values with those of authentic compounds and were mg seed of Phaseolus rnultiflorus. Previous investiga­ c~nfirmed by determination of fluorescence spectra'. 6 12 tions"-' of the gibberdlins of developing seeds have been G1bberellms A A , As and A are known • constituents 10 5 8 made using paper chromatography in conjunction with of rmmer bean seeds, but gibberellin A has not hitherto bioassay, which does not allow of positive identification http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nature Springer Journals

Examination of the Gibberellins of Zea mays and Phaseolus multiflorus Using Thin-Layer Chromatography

JONES, D. F.
Nature , Volume 202 (4939) – Jun 27, 1964
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References (14)

Publisher
Springer Journals
Copyright
Copyright © 1964 by Nature Publishing Group
Subject
Science, Humanities and Social Sciences, multidisciplinary; Science, Humanities and Social Sciences, multidisciplinary; Science, multidisciplinary
ISSN
0028-0836
eISSN
1476-4687
DOI
10.1038/2021309a0
Publisher site
See Article on Publisher Site

Abstract

No. 4939 June 27, 1964 NATURE EXAMINATION OF THE GIBBERELLINS OF Zea mays AND Phaseolus multiflorus USING THIN-LAYER CHROMATOGRAPHY By D. F. JONES Imperial Chemical Industries, Ltd., Pharmaceutical Division, Akers Research Laboratories, Welwyn 1 2 REVIOUS publications • have described the identifica­ tied by their response to ethanolic sulphuric acid detection tion of microgramme amounts of the endogenous and by the characteristic migratory properties'·'·7 of gibberellins of some higher plant seeds by thin-layer the free acids and their derived methyl esters on thin­ chromatography. This technique has now been used to laye_r chromato~rap~y in the systems listed in the previous identify gibberellin A in an extract of Zea mays seed sectwn. Ident1ficatwns were made by direct comparison :<nd in quantitative analysis of the gibberellins in develop­ of RF values with those of authentic compounds and were mg seed of Phaseolus rnultiflorus. Previous investiga­ c~nfirmed by determination of fluorescence spectra'. 6 12 tions"-' of the gibberdlins of developing seeds have been G1bberellms A A , As and A are known • constituents 10 5 8 made using paper chromatography in conjunction with of rmmer bean seeds, but gibberellin A has not hitherto bioassay, which does not allow of positive identification

Journal

Nature – Springer Journals

Published: Jun 27, 1964

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