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<i>Drosophila</i> homologs of transcriptional mediator complex subunits are required for adult cell and segment identity specification

Drosophila homologs of transcriptional mediator complex subunits are required for adult... Downloaded from genesdev.cshlp.org on November 16, 2021 - Published by Cold Spring Harbor Laboratory Press Drosophila homologs of transcriptional mediator complex subunits are required for adult cell and segment identity specification 1 2 Muriel Boube, Christian Faucher, Laurent Joulia, David L. Cribbs, and Henri-Marc Bourbon Centre de Biologie du De ´ veloppement-CNRS, 31062 Toulouse CEDEX 04, France The origins of specificity in gene expression are a central concern in understanding developmental control. Mediator protein complexes regulate transcriptional initiation, acting as modular adaptors linking specific transcription factors to core RNA polymerase II. Here, we identified the Drosophila homologs of 23 human mediator genes and mutations of two, dTRAP240 and of dTRAP80 (the putative fly homolog of yeast SRB4). Clonal analysis indicates a general role for dTRAP80 necessary for cell viability. The dTRAP240 gene is also essential, but cells lacking its function are viable and proliferate normally. Clones reveal localized developmental activities including a sex comb cell identity function. This contrasts with the ubiquitous nuclear accumulation of dTRAP240 protein in imaginal discs. Synergistic genetic interactions support shared developmental cell and segment identity functions of dTRAP240 and dTRAP80, potentially within a common complex. Further, they identify the homeotic Sex combs reduced product, http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Genes & Development Unpaywall

<i>Drosophila</i> homologs of transcriptional mediator complex subunits are required for adult cell and segment identity specification

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Unpaywall
ISSN
0890-9369
DOI
10.1101/gad.17900
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Abstract

Downloaded from genesdev.cshlp.org on November 16, 2021 - Published by Cold Spring Harbor Laboratory Press Drosophila homologs of transcriptional mediator complex subunits are required for adult cell and segment identity specification 1 2 Muriel Boube, Christian Faucher, Laurent Joulia, David L. Cribbs, and Henri-Marc Bourbon Centre de Biologie du De ´ veloppement-CNRS, 31062 Toulouse CEDEX 04, France The origins of specificity in gene expression are a central concern in understanding developmental control. Mediator protein complexes regulate transcriptional initiation, acting as modular adaptors linking specific transcription factors to core RNA polymerase II. Here, we identified the Drosophila homologs of 23 human mediator genes and mutations of two, dTRAP240 and of dTRAP80 (the putative fly homolog of yeast SRB4). Clonal analysis indicates a general role for dTRAP80 necessary for cell viability. The dTRAP240 gene is also essential, but cells lacking its function are viable and proliferate normally. Clones reveal localized developmental activities including a sex comb cell identity function. This contrasts with the ubiquitous nuclear accumulation of dTRAP240 protein in imaginal discs. Synergistic genetic interactions support shared developmental cell and segment identity functions of dTRAP240 and dTRAP80, potentially within a common complex. Further, they identify the homeotic Sex combs reduced product,

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Genes & Development – Unpaywall

Published: Nov 15, 2000

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