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Invasion of Lymphosarcoma Cells Into the Perfused Mouse Liver

Invasion of Lymphosarcoma Cells Into the Perfused Mouse Liver Abstract Livers of (C57BL × DBA)F1 mice were perfused in situ with a synthetic hemoglobin-free medium, to which murine lymphosarcoma cells were added. All cells were arrested. They were only found in the sinusoids, predominantly in periportal areas. Many lymphosarcoma cells penetrated the walls of the sinusoids with protrusions that extended into and through the endothelial cells. The protrusions often also invaded hepatocytes, and some cells migrated out of the sinusoids. The percentage of the cells that penetrated endothelium was constant and reproducible (68±4%) in experiments lasting longer than 90 minutes, but the percentage of these cells that also invaded hepatocytes varied greatly. Parallel experiments in vivo yielded similar results, except that the number of cells that invaded hepatocytes was generally much lower. The advantages of the perfused liver as a model for experimental study of the invasion mechanism were evaluated. This content is only available as a PDF. Author notes 2 Division of Cell Biology, Antoni van Leeuwenhoekhuis, The Netherlands Cancer Institute, Sarphatistraat 108, Amsterdam, The Netherlands. 3 Laboratory for Pathological Anatomy, Wilhelmina Gasthuis, Ie Helmersstraat 104, Amsterdam, The Netherlands.s 4 We are indebted to Miss C. Koning for preparing the micrographs. We thank Dr. C. A. Feltkamp and Dr.]. H. M. Temmink for help in preparing the manuscript, and Dr. L. A. Smets, Mr. C. Homburg, Dr. C. Benckhuysen, and Mr. E. Spanjersberg for providing cells and facilities. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png JNCI: Journal of the National Cancer Institute Oxford University Press

Invasion of Lymphosarcoma Cells Into the Perfused Mouse Liver

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Publisher
Oxford University Press
ISSN
0027-8874
eISSN
1460-2105
DOI
10.1093/jnci/58.2.399
Publisher site
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Abstract

Abstract Livers of (C57BL × DBA)F1 mice were perfused in situ with a synthetic hemoglobin-free medium, to which murine lymphosarcoma cells were added. All cells were arrested. They were only found in the sinusoids, predominantly in periportal areas. Many lymphosarcoma cells penetrated the walls of the sinusoids with protrusions that extended into and through the endothelial cells. The protrusions often also invaded hepatocytes, and some cells migrated out of the sinusoids. The percentage of the cells that penetrated endothelium was constant and reproducible (68±4%) in experiments lasting longer than 90 minutes, but the percentage of these cells that also invaded hepatocytes varied greatly. Parallel experiments in vivo yielded similar results, except that the number of cells that invaded hepatocytes was generally much lower. The advantages of the perfused liver as a model for experimental study of the invasion mechanism were evaluated. This content is only available as a PDF. Author notes 2 Division of Cell Biology, Antoni van Leeuwenhoekhuis, The Netherlands Cancer Institute, Sarphatistraat 108, Amsterdam, The Netherlands. 3 Laboratory for Pathological Anatomy, Wilhelmina Gasthuis, Ie Helmersstraat 104, Amsterdam, The Netherlands.s 4 We are indebted to Miss C. Koning for preparing the micrographs. We thank Dr. C. A. Feltkamp and Dr.]. H. M. Temmink for help in preparing the manuscript, and Dr. L. A. Smets, Mr. C. Homburg, Dr. C. Benckhuysen, and Mr. E. Spanjersberg for providing cells and facilities.

Journal

JNCI: Journal of the National Cancer Institute – Oxford University Press

Published: Feb 1, 1977

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