Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 7-Day Trial for You or Your Team.

Learn More →

Biosensors and BiodetectionSurface Plasmon Resonance Biosensor for Biomolecular Interaction Analysis Based on Spatial Modulation Phase Detection

Biosensors and Biodetection: Surface Plasmon Resonance Biosensor for Biomolecular Interaction... [Surface plasmon resonance (SPR) biosensor is a powerful tool for biomolecular interaction analysis in proteomics research and drug discovery. But when it is used to analyze small molecules, the sensitivity still needs enhancement. Phase detection is a potential solution, for phase changes more abruptly than intensity when SPR is excited. An SPR system is developed based on spatial modulation phase detection (SMPD). In this system, collimated monochromatic light is used to excite SPR, and the phase of the reflected light is spatially modulated to generate an interference pattern. By processing the interference pattern by certain algorithms, instantaneous phase distribution of the whole sensing area can be obtained. Continuously detecting the phase change, the whole process of biomolecular interaction can be recorded in the form of phase change, based on which we can make kinetic analysis and get kinetic parameters. Antibody array chip is tested by this system. Experimental results indicate that this technique is capable of array detection and is more sensitive than intensity detection.] http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

Biosensors and BiodetectionSurface Plasmon Resonance Biosensor for Biomolecular Interaction Analysis Based on Spatial Modulation Phase Detection

Part of the Methods in Molecular Biology™ Book Series (volume 503)
Editors: Rasooly, Avraham; Herold, Keith E.
Ding, Xiang; Liu, Fangfang; Yu, Xinglong
Biosensors and Biodetection — Jan 1, 2009
Download PDF
15 pages

Loading next page...
 
/lp/springer-journals/biosensors-and-biodetection-surface-plasmon-resonance-biosensor-for-gxOBTi0otx

References (4)

Publisher
Humana Press
Copyright
© Humana Press 2009
ISBN
978-1-60327-566-8
Pages
21–35
DOI
10.1007/978-1-60327-567-5_2
Publisher site
See Chapter on Publisher Site

Abstract

[Surface plasmon resonance (SPR) biosensor is a powerful tool for biomolecular interaction analysis in proteomics research and drug discovery. But when it is used to analyze small molecules, the sensitivity still needs enhancement. Phase detection is a potential solution, for phase changes more abruptly than intensity when SPR is excited. An SPR system is developed based on spatial modulation phase detection (SMPD). In this system, collimated monochromatic light is used to excite SPR, and the phase of the reflected light is spatially modulated to generate an interference pattern. By processing the interference pattern by certain algorithms, instantaneous phase distribution of the whole sensing area can be obtained. Continuously detecting the phase change, the whole process of biomolecular interaction can be recorded in the form of phase change, based on which we can make kinetic analysis and get kinetic parameters. Antibody array chip is tested by this system. Experimental results indicate that this technique is capable of array detection and is more sensitive than intensity detection.]

Published: Jan 1, 2009

Keywords: Surface plasmon resonance (SPR); Spatial modulation; Phase detection; Biomolecular interaction; Antibody array

There are no references for this article.