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References (33)
-
M. Hunkapiller, L. Hood (1983)
Protein sequence analysis: automated microsequencing.Science, 219 4585
-
A. Shevchenko, M. Wilm, O. Vorm, Millard Mann (1996)
Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels.Analytical chemistry, 68 5
-
P. O’Farrell (1975)
High resolution two-dimensional electrophoresis of proteins.The Journal of biological chemistry, 250 10
-
E. Gianazza, F. Celentano, Stefania Magenes, C. Ettori, P. Righetti (1989)
Formulations for immobilized pH gradients including pH extremesELECTROPHORESIS, 10
-
A. Görg, G. Boguth, C. Obermaier, A. Posch, W. Weiss (1995)
Two‐dimensional polyacrylamide gel electrophoresis with immobilized pH gradients in the first dimensin (IPG‐Dalt): The state of the art and the controversy of vertical versus horizontal systemsELECTROPHORESIS, 16
-
H. Rasmussen, E. Mørtz, M. Mann, P. Roepstorff, J. Celis (1994)
Identification of transformation sensitive proteins recorded in human two‐dimensional gel protein databases by mass spectrometric peptide mapping alone and in combination with microsequencingELECTROPHORESIS, 15
-
D. Pappin, P. Højrup, A. Bleasby (1993)
Rapid identification of proteins by peptide-mass fingerprintingCurrent Biology, 3
-
(1994)
Prize Project: Faculty of -
M. Wilkins, K. Ou, R. Appel, J. Sánchez, J. Yan, O. Golaz, V. Farnsworth, P. Cartier, D. Hochstrasser, K. Williams, A. Gooley (1996)
Rapid protein identification using N-terminal "sequence tag" and amino acid analysis.Biochemical and biophysical research communications, 221 3
-
O. Jensen, A. Podtelejnikov, M. Mann (1996)
Delayed extraction improves specificity in database searches by matrix-assisted laser desorption/ionization peptide maps.Rapid communications in mass spectrometry : RCM, 10 11
-
A. Görg (1991)
Two-dimensional electrophoresisNature, 349
-
A. Görg, W. Postel, S. Günther (1988)
Two‐dimensional electrophoresis. The current state of two‐dimensional electrophoresis with immobilized pH gradientsELECTROPHORESIS, 9
-
A. Blomberg (1997)
Osmoresponsive proteins and functional assessment strategies in Saccharomyces cerevisiaeELECTROPHORESIS, 18
-
A. Blomberg, L. Blomberg, J. Norbeck, S. Fey, P. Larsen, M. Larsen, P. Roepstorff, H. Degand, M. Boutry, A. Posch, A. Görg (1995)
Interlaboratory reproducibility of yeast protein patterns analyzed by immobilized pH gradient two‐dimensional gel electrophoresisELECTROPHORESIS, 16
-
(1984)
Prize Project: Faculty National Science, Aarhus University, Aarhus -
J. Garrels, B. Futcher, R. Kobayashi, G. Latter, Brian Schwender, T. Volpe, J. Warner, C. McLaughlin (1994)
Protein identifications for a Saccharomyces cerevisiae protein databaseELECTROPHORESIS, 15
-
of carrier ampholyte and IPG generated 2-D patterns 1035 -
M. Bradford (1976)
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Analytical biochemistry, 72
-
J. Yates, S. Speicher, P. Griffin, T. Hunkapiller (1993)
Peptide mass maps: a highly informative approach to protein identification.Analytical biochemistry, 214 2
-
P. O'Farrell, H. Goodman, P. O’Farrell (1977)
High resolution two-dimensional electrophoresis of basic as well as acidic proteinsCell, 12
-
J. Rosenfeld, J. Capdevielle, J. Guillemot, P. Ferrara (1992)
In-gel digestion of proteins for internal sequence analysis after one- or two-dimensional gel electrophoresis.Analytical biochemistry, 203 1
-
Jean‐Charles Sanchez, O. Golaz, S. Frutiger, Dominique Schaller, R. Appel, A. Bairoch, G. Hughes, D. Hochstrasser (1996)
The yeast SWISS‐2DPAGE databaseELECTROPHORESIS, 17
-
H. Boucherie, C. Monribot, M. Perrot, G. Dujardin, M. Kermorgant, P. Slonimski (1995)
Two‐Dimensional protein map of Saccharomyces cerevisiae: Construction of a gene–protein indexYeast, 11
-
(1991)
Electrophoresis 1988 -
O. Jensen, and Podtelejnikov, Matthias Mann (1997)
Identification of the components of simple protein mixtures by high-accuracy peptide mass mapping and database searching.Analytical chemistry, 69 23
-
S. Fey, A. Nawrocki, M. Larsen, A. Görg, P. Roepstorff, Gerard Skews, R. Williams, P. Larsen (1997)
Proteome analysis of Saccharomyces cerevisiae: A methodological outlineELECTROPHORESIS, 18
-
O. Vorm, P. Roepstorff, M. Mann (1994)
Improved resolution and very high sensitivity in MALDI TOF of matrix surfaces made by fast evaporationAnalytical Chemistry, 66
-
P. James, M. Quadroni, E. Carafoli, G. Gonnet (1993)
Protein identification by mass profile fingerprinting.Biochemical and biophysical research communications, 195 1
-
M. Mann, P. Højrup, P. Roepstorff (1993)
Use of mass spectrometric molecular weight information to identify proteins in sequence databases.Biological mass spectrometry, 22 6
-
A. Shevchenko, O. Jensen, A. Podtelejnikov, F. Sagliocco, M. Wilm, O. Vorm, P. Mortensen, A. Shevchenko, H. Boucherie, M. Mann (1996)
Linking genome and proteome by mass spectrometry: large-scale identification of yeast proteins from two dimensional gels.Proceedings of the National Academy of Sciences of the United States of America, 93 25
-
M. Kussmann, E. Nordhoff, H. Rahbek-Nielsen, S. Haebel, Martin Røssel-Larsen, L. Jakobsen, J. Gobom, E. Mirgorodskaya, Anne Kroll-Kristensen, L. Palm, P. Roepstorff (1997)
Matrix‐assisted Laser Desorption/Ionization Mass Spectrometry Sample Preparation Techniques Designed for Various Peptide and Protein AnalytesJournal of Mass Spectrometry, 32
-
O. Jensen, P. Mortensen, O. Vorm, Matthias Mann (1997)
Automation of matrix-assisted laser desorption/ionization mass spectrometry using fuzzy logic feedback control.Analytical chemistry, 69 9
-
W. Henzel, T. Billeci, J. Stults, Susan Wong, C. Grimley, C. Watanabe (1993)
Identifying proteins from two-dimensional gels by molecular mass searching of peptide fragments in protein sequence databases.Proceedings of the National Academy of Sciences of the United States of America, 90 11
- Publisher
- Wiley
- Copyright
- "Copyright © 1998 Wiley Subscription Services, Inc., A Wiley Company"
- ISSN
- 0173-0835
- eISSN
- 1522-2683
- DOI
- 10.1002/elps.1150190618
- pmid
- 9638949
- Publisher site
- See Article on Publisher Site
Abstract
Separation of proteins on either carrier ampholyte‐based or immobilized pH gradient‐based two‐dimensional (2‐D) gels gives rise to electrophoretic patterns that are difficult to compare visually. In this paper we have used matrix‐assisted laser desorption/ionization mass spectrometry (MALDI‐MS) to determine the identities of 335 protein spots in these two 2‐D gel systems, including a substantial number of basic proteins which had never been identified before. Proteins that were identified in both gel systems allowed us to cross‐reference the gel patterns. Vector analysis of these cross‐references demonstrated that there is no obvious pattern by which the mobility of a protein in one gel system can be used to predict its mobility in the other. Thus, as laboratories adopt the immobilized pH gradient‐based 2‐D gel systems, the only reliable means of translating the data gained with the carrier ampholyte‐based gel system is to positively identify the proteins in both 2‐D systems.
Journal
Electrophoresis – Wiley
Published: May 1, 1998
Keywords: ;