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/lp/karger/influence-of-temperature-on-the-determination-of-enzyme-activities-in-ggazoQCxqE
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- Publisher
- Karger
- Copyright
- © 1977 S. Karger AG, Basel
- ISSN
- 0013-9432
- eISSN
- 2504-2564
- DOI
- 10.1159/000458792
- Publisher site
- See Article on Publisher Site
Abstract
Optimum reaction conditions for determination of 7-glutamyl transferase werestudied at 25, 30 and 37 °C using a kinetic test and 7-glutamyl-3-carboxy-4-nitranilide as substrate.There was no dependence on temperature for half saturation constants of 7-glutamyl-3-carboxy-4-nitranilide. The corresponding constants for glycylglycine were influenced by temperature andthe pH. The pH optimum showed a dependence upon temperature. In Arrhenius’ plot, a deviationfrom straight line can be observed only above 35 °C. The influence of temperature on thedetermination of enzyme activities in human serum are discussed.
Journal
Enzyme – Karger
Published: Jan 1, 1977
Keywords: Human serum; γ-Glutamyl transferase; γ-Glutamyl-3-carboxy-4-nitranilide as substrate; Optimum reaction conditions; Influence of temperature