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Cohesin release is required for sister chromatid resolution, but not for condensin-mediated compaction, at the onset of mitosis

Cohesin release is required for sister chromatid resolution, but not for condensin-mediated... Downloaded from genesdev.cshlp.org on December 3, 2021 - Published by Cold Spring Harbor Laboratory Press Cohesin release is required for sister chromatid resolution, but not for condensin-mediated compaction, at the onset of mitosis Ana Losada, Michiko Hirano, and Tatsuya Hirano Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA The establishment of metaphase chromosomes is an essential prerequisite of sister chromatid separation in anaphase. It involves the coordinated action of cohesin and condensin, protein complexes that mediate cohesion and condensation, respectively. In metazoans, most cohesin dissociates from chromatin at prophase, coincident with association of condensin. Whether loosening of cohesion at the onset of mitosis facilitates the compaction process, resolution of the sister chromatids, or both, remains unknown. We have found that the prophase release of cohesin is completely blocked when two mitotic kinases, aurora B and polo-like kinase (Plx1), are simultaneously depleted from Xenopus egg extracts. Condensin loading onto chromatin is not affected under this condition, and rod-shaped chromosomes are produced that show an apparently normal level of compaction. However, the resolution of sister chromatids within these chromosomes is severely compromised. This is not because of inhibition of topoisomerase II activity that is also required for the http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Genes & Development Unpaywall

Cohesin release is required for sister chromatid resolution, but not for condensin-mediated compaction, at the onset of mitosis

Losada, Ana; Hirano, Michiko; Hirano, Tatsuya
Genes & Development – Dec 1, 2002
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Unpaywall
ISSN
0890-9369
DOI
10.1101/gad.249202
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Abstract

Downloaded from genesdev.cshlp.org on December 3, 2021 - Published by Cold Spring Harbor Laboratory Press Cohesin release is required for sister chromatid resolution, but not for condensin-mediated compaction, at the onset of mitosis Ana Losada, Michiko Hirano, and Tatsuya Hirano Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA The establishment of metaphase chromosomes is an essential prerequisite of sister chromatid separation in anaphase. It involves the coordinated action of cohesin and condensin, protein complexes that mediate cohesion and condensation, respectively. In metazoans, most cohesin dissociates from chromatin at prophase, coincident with association of condensin. Whether loosening of cohesion at the onset of mitosis facilitates the compaction process, resolution of the sister chromatids, or both, remains unknown. We have found that the prophase release of cohesin is completely blocked when two mitotic kinases, aurora B and polo-like kinase (Plx1), are simultaneously depleted from Xenopus egg extracts. Condensin loading onto chromatin is not affected under this condition, and rod-shaped chromosomes are produced that show an apparently normal level of compaction. However, the resolution of sister chromatids within these chromosomes is severely compromised. This is not because of inhibition of topoisomerase II activity that is also required for the

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Genes & Development – Unpaywall

Published: Dec 1, 2002

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