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Evidence suggesting that L‐fucose is part of a recognition signal for sperm‐zona pellucida attachment in mammals

Evidence suggesting that L‐fucose is part of a recognition signal for sperm‐zona pellucida... Experiments were designed to test the effects of simple sugars and complex polysaccharides on the attachment of mammalian spermatozoa with the zona pellucida. In the guinea pig, L‐fucose was a twofold better inhibitor of the attachment compared to other sugars at 50 mM. Fucoidin, an algal polysaccharide rich in sulfated L‐fucose, was a very potent inhibitor, completely blocking attachment at a concentration of 100 μg/ml. Several other highly sulfated glycosaminoglycans showed no inhibitory activity, suggesting the fucoidin effect was not simply due to its charge or sulfate. In addition, fragments of fucoidin, generated by partial hydrolysis and isolated using Biogel P‐2, were nearly as inhibitory as the native molecule on a weight basis. Fucoidin also inhibited sperm‐zona attachment in the hamster and human; thus, its effect is not species specific. The data suggest that L‐fucose may be part of a recognition signal between mammalian gametes. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Molecular Reproduction & Development Wiley

Evidence suggesting that L‐fucose is part of a recognition signal for sperm‐zona pellucida attachment in mammals

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References (25)

Publisher
Wiley
Copyright
Copyright © 1982 Wiley Subscription Services, Inc., A Wiley Company
ISSN
1040-452X
eISSN
1098-2795
DOI
10.1002/mrd.1120050406
Publisher site
See Article on Publisher Site

Abstract

Experiments were designed to test the effects of simple sugars and complex polysaccharides on the attachment of mammalian spermatozoa with the zona pellucida. In the guinea pig, L‐fucose was a twofold better inhibitor of the attachment compared to other sugars at 50 mM. Fucoidin, an algal polysaccharide rich in sulfated L‐fucose, was a very potent inhibitor, completely blocking attachment at a concentration of 100 μg/ml. Several other highly sulfated glycosaminoglycans showed no inhibitory activity, suggesting the fucoidin effect was not simply due to its charge or sulfate. In addition, fragments of fucoidin, generated by partial hydrolysis and isolated using Biogel P‐2, were nearly as inhibitory as the native molecule on a weight basis. Fucoidin also inhibited sperm‐zona attachment in the hamster and human; thus, its effect is not species specific. The data suggest that L‐fucose may be part of a recognition signal between mammalian gametes.

Journal

Molecular Reproduction & Development – Wiley

Published: Jan 1, 1982

Keywords: ; ; ; ;

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