Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 7-Day Trial for You or Your Team.

Learn More →

Genetic Analysis of Flagellar Length Control in Chlamydomonas reinhardtii: A New Long-Flagella Locus and Extragenic Suppressor Mutations

Genetic Analysis of Flagellar Length Control in Chlamydomonas reinhardtii: A New Long-Flagella... Flagellar length in the biflagellate alga Chlamydomonas reinhardtii is under constant and tight regulation. A number of mutants with defects in flagellar length control have been previously identified. Mutations in the three long-flagella (lf) loci result in flagella that are up to three times longer than wild-type length. In this article, we describe the isolation of long-flagellar mutants caused by mutations in a new LF locus, LF4. lf4 mutations were shown to be epistatic to lf1, while lf2 was found to be epistatic to lf4 with regard to the flagellar regeneration defect. Mutations in lf4 were able to suppress the synthetic flagella-less phenotype of the lf1, lf2 double mutant. In addition, we have isolated four extragenic suppressor mutations that suppress the long-flagella phenotype of lf1, lf2, or lf3 double mutants. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Genetics Oxford University Press

Genetic Analysis of Flagellar Length Control in Chlamydomonas reinhardtii: A New Long-Flagella Locus and Extragenic Suppressor Mutations

Asleson, Catherine M; Lefebvre, Paul A
Genetics , Volume 148 (2): 10 – Feb 1, 1998
Download PDF
10 pages

Loading next page...
 
/lp/oxford-university-press/genetic-analysis-of-flagellar-length-control-in-chlamydomonas-dEco4XMyTC

References (10)

Publisher
Oxford University Press
Copyright
© Genetics 1998
ISSN
0016-6731
eISSN
1943-2631
DOI
10.1093/genetics/148.2.693
Publisher site
See Article on Publisher Site

Abstract

Flagellar length in the biflagellate alga Chlamydomonas reinhardtii is under constant and tight regulation. A number of mutants with defects in flagellar length control have been previously identified. Mutations in the three long-flagella (lf) loci result in flagella that are up to three times longer than wild-type length. In this article, we describe the isolation of long-flagellar mutants caused by mutations in a new LF locus, LF4. lf4 mutations were shown to be epistatic to lf1, while lf2 was found to be epistatic to lf4 with regard to the flagellar regeneration defect. Mutations in lf4 were able to suppress the synthetic flagella-less phenotype of the lf1, lf2 double mutant. In addition, we have isolated four extragenic suppressor mutations that suppress the long-flagella phenotype of lf1, lf2, or lf3 double mutants.

Journal

Genetics – Oxford University Press

Published: Feb 1, 1998

There are no references for this article.