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Development of a chemiluminescence detection system using bis(4‐nitro‐2‐(3,6,9‐trioxadecyloxycarbonyl)phenyl) oxalate for the sensitive determination of the fluorescent compounds separated with an acidic mobile phase

Development of a chemiluminescence detection system using... A rotating flow mixing device having three directional inlets was developed for a high performance liquid chromatographic peroxyoxalate chemiluminescence detection system for the sensitive detection of fluorescent compounds separated with acidic mobile phases, such as those containing trifluoroacetic acid. The diameters of the three inlets were 0.3, 0.3 and 0.5 mm i.d., for an acidic column eluate, chemiluminogenic reagents (bis(4‐nitro‐2‐(3,6,9‐trioxadecyloxycarbonyl)phenyl) oxalate and hydrogen peroxide) and imidazole solution (for neutralization and as a catalyst), respectively. The three mixed solutions flowed out through an outlet (0.5 mm i.d.) at the centre of the top of the vessel. The reaction conditions to give the appropriate signal‐to‐noise ratio (S/N) were investigated using dipyridamole (an antiplatelet aggregation drug) as a typical fluorescent compound. The system was applied to the detection of dipyridamole and dansylated amino acids with a mobile phase containing trifluoroacetic acid. The detection limits for dipyridamole and each dansylated amino acid were 10 amol and sub‐fmol (S/N=2), respectively. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biomedical Chromatography Wiley

Development of a chemiluminescence detection system using bis(4‐nitro‐2‐(3,6,9‐trioxadecyloxycarbonyl)phenyl) oxalate for the sensitive determination of the fluorescent compounds separated with an acidic mobile phase

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References (27)

Publisher
Wiley
Copyright
Copyright © 1993 John Wiley & Sons, Ltd.
ISSN
0269-3879
eISSN
1099-0801
DOI
10.1002/bmc.1130070310
pmid
8318832
Publisher site
See Article on Publisher Site

Abstract

A rotating flow mixing device having three directional inlets was developed for a high performance liquid chromatographic peroxyoxalate chemiluminescence detection system for the sensitive detection of fluorescent compounds separated with acidic mobile phases, such as those containing trifluoroacetic acid. The diameters of the three inlets were 0.3, 0.3 and 0.5 mm i.d., for an acidic column eluate, chemiluminogenic reagents (bis(4‐nitro‐2‐(3,6,9‐trioxadecyloxycarbonyl)phenyl) oxalate and hydrogen peroxide) and imidazole solution (for neutralization and as a catalyst), respectively. The three mixed solutions flowed out through an outlet (0.5 mm i.d.) at the centre of the top of the vessel. The reaction conditions to give the appropriate signal‐to‐noise ratio (S/N) were investigated using dipyridamole (an antiplatelet aggregation drug) as a typical fluorescent compound. The system was applied to the detection of dipyridamole and dansylated amino acids with a mobile phase containing trifluoroacetic acid. The detection limits for dipyridamole and each dansylated amino acid were 10 amol and sub‐fmol (S/N=2), respectively.

Journal

Biomedical Chromatography – Wiley

Published: May 1, 1993

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