Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 7-Day Trial for You or Your Team.

Learn More →

BACK-FLUSH COLUMN-SWITCHING TECHNIQUE FOR ON-LINE SAMPLE CLEANUP AND ENRICHMENT TO DETERMINE GUAIPHENESIN IN HUMAN SERUM

BACK-FLUSH COLUMN-SWITCHING TECHNIQUE FOR ON-LINE SAMPLE CLEANUP AND ENRICHMENT TO DETERMINE... A direct injection high-performance liquid chromatographic (HPLC) method, with column-switching, for the determination of guaiphenesin (GP) in human serum has been developed. Serum samples were directly injected onto protein-coated RP-8 silica precolumn, where GP was preconcentrated and retained while proteins and very polar components were washed to waste using phosphate buffer saline, pH 7.4. GP was back-flushed from the precolumn by a column-switching technique and separated on a ZORBAX Eclipse XDB-C18 analytical column with a mobile phase consisting of methanol-0.01 M phosphate buffer (containing 1% triethylamine adjusted to pH 3.5 with ortho-phosphoric acid) in the ratio of 45:55 (v/v). The analyte was detected by its UV absorbance at 254 nm. The calibration curve was linear over the concentration range of 25–4000 ng/mL ( r 2 = 0.9994). The method was validated for linearity, accuracy, precision, selectivity, and robustness. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Liquid Chromatography & Related Technologies Taylor & Francis

BACK-FLUSH COLUMN-SWITCHING TECHNIQUE FOR ON-LINE SAMPLE CLEANUP AND ENRICHMENT TO DETERMINE GUAIPHENESIN IN HUMAN SERUM

Download PDF
13 pages

Loading next page...
 
/lp/taylor-francis/back-flush-column-switching-technique-for-on-line-sample-cleanup-and-ci2p3ZVZas

References (11)

Publisher
Taylor & Francis
Copyright
Copyright Taylor & Francis Group, LLC
ISSN
1520-572X
eISSN
1082-6076
DOI
10.1080/10826076.2011.593226
Publisher site
See Article on Publisher Site

Abstract

A direct injection high-performance liquid chromatographic (HPLC) method, with column-switching, for the determination of guaiphenesin (GP) in human serum has been developed. Serum samples were directly injected onto protein-coated RP-8 silica precolumn, where GP was preconcentrated and retained while proteins and very polar components were washed to waste using phosphate buffer saline, pH 7.4. GP was back-flushed from the precolumn by a column-switching technique and separated on a ZORBAX Eclipse XDB-C18 analytical column with a mobile phase consisting of methanol-0.01 M phosphate buffer (containing 1% triethylamine adjusted to pH 3.5 with ortho-phosphoric acid) in the ratio of 45:55 (v/v). The analyte was detected by its UV absorbance at 254 nm. The calibration curve was linear over the concentration range of 25–4000 ng/mL ( r 2 = 0.9994). The method was validated for linearity, accuracy, precision, selectivity, and robustness.

Journal

Journal of Liquid Chromatography & Related Technologies – Taylor & Francis

Published: Jan 1, 2012

Keywords: column-switching; guaiphenesin; HPLC; protein-coated RP-8 silica precolumn; ZORBAX Eclipse XDB-C18 column

There are no references for this article.