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Establishment of a Simple and Rapid Identification Method for Listeria spp. by Using High-Resolution Melting Analysis, and Its Application in Food Industry

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http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png PLoS ONE Pubmed Central

Establishment of a Simple and Rapid Identification Method for Listeria spp. by Using High-Resolution Melting Analysis, and Its Application in Food Industry

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Publisher
Pubmed Central
Copyright
© 2014 Ohshima et al
ISSN
1932-6203
eISSN
1932-6203
DOI
10.1371/journal.pone.0099223
Publisher site
See Article on Publisher Site

Abstract

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

Journal

PLoS ONE – Pubmed Central

Published: Jun 11, 2014

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