Access the full text.
Sign up today, get DeepDyve free for 14 days.
Loading next page...
/lp/springer-journals/regeneration-of-transgenic-vegetable-brassicas-brassica-oleracea-and-b-cZgNDH30qN
References (22)
-
MC Christey, BK Sinclair (1992)
Regeneration of transgenic kale (Brassica oleracea var.acephala), rape (B. napus) and turnip (B. campestris var.rapifera) plants viaAgrobacterium rhizogenes mediated transformationPlant Sci, 87
-
MW Lassner, P Peterson, JI Yoder (1989)
Simultaneous amplification of multiple DNA fragments by polymerase chain reaction in the analysis of transgenic plants and their progenyPlant Mol Biol Rep, 7
-
JE Grant, EM Dommisse, MC Christey, AJ Conner (1991)
Advanced methods in plant breeding and biotechnology -
C David, J Tempe (1988)
Genetic transformation of cauliflower (Brassica oleracea L. var.botrytis) byAgrobacterium rhizogenesPlant Cell Rep, 7
-
EM Linsmaier, F Skoog (1965)
Organic growth factor requirements of tobacco tissue culturesPhysiol Plant, 18
-
MC Christey (1997)
Hairy roots: culture and applications -
J. Grant, E. Dommisse, M. Christey, A. Conner, D. Murray (1991)
Gene transfer to plants using Agrobacterium. -
G An, PR Ebert, A Mitra, SB Ha (1988)
Plant molecular biology manual -
D Tepfer (1989)
Plant-microbe interactions. Molecular and genetic perspectives, vol 3 -
C Montanelli, G Nascari (1991)
Introduction of an antibacterial gene in potato (Solanum tuberosum L.) using a binary vector inAgrobacterium rhizogenesJ Genet Breed, 45
-
P Guerche, L Jouanin, D Tepfer, G Pelletier (1987)
Genetic transformation of oilseed rape (Brassica napus) by the Ri T-DNA ofAgrobacterium rhizogenes and analysis of inheritance of the transformed phenotypeMol Gen Genet, 206
-
P Berthomieu, L Jouanin (1992)
Transformation of rapid cycling cabbage (Brassica oleracea var.capitata) withAgrobacterium rhizogenesPlant Cell Rep, 11
-
H Hatamoto, ME Boulter, AH Shirsat, EJ Croy, JR Ellis (1990)
Recovery of morphologically normal transgenic tobacco from hairy roots co-transformed withAgrobacterium rhizogenes and a binary vector plasmidPlant Cell Rep, 9
-
AP Gleave (1992)
A versatile binary vector system with a T-DNA organisational structure conducive to efficient integration of cloned DNA into the plant genomePlant Mol Biol, 20
-
O Damgaard, O Rasmussen (1991)
Direct regeneration of transformed shoots inBrassica napus from hypocotyl infections withAgrobacterium rhizogenesPlant Mol Biol, 17
-
L Moore, G Warren, G Strobel (1979)
Involvement of a plasmid in the hairy root disease of plants caused byAgrobacterium rhizogenesPlasmid, 2
-
SI Jun, SY Kwon, KY Paek, KH Paek (1995)
Agrobacterium-mediated transformation and regeneration of fertile transgenic plants of chinese cabbage (Brassica campestris spp.pekinensis cv. “Spring Flavor”)Plant Cell Rep, 14
-
ME Boulter, E Croy, P Simpson, R Shields, RRD Croy, AH Shirsat (1990)
Transformation ofBrassica napus L. (oilseed rape) usingAgrobacterium tumefaciens andAgrobacterium rhizogenes — a comparisonPlant Sci, 70
-
GM Timmerman, TJ Frew, AL Miller, NF Weedon, WA Jermyn (1993)
Linkage mapping ofsbm-1, a gene conferring resistance to pea seed-borne mosaic virus, using molecular markers inPisum sativumTheor Appl Genet, 85
-
T Noda, N Tanaka, Y Mano, S Nabeshima, H Ohkawa, C Matsui (1987)
Regeneration of horseradish hairy roots incited byAgrobacterium rhizogenes infectionPlant Cell Rep, 6
-
MD Burow, CA Chlan, P Sen, A Lisca, N Murai (1990)
High-frequency generation of transgenic tobacco plants after modified leaf disc cocultivation withAgrobacterium tumefaciensPlant Mol Biol Rep, 8
-
KJ Webb, S Jones, MP Robbins, FR Minchin (1990)
Characterization of transgenic root cultures ofTrifolium repens, Trifolium pratense andLotus corniculatus and transgenic plants ofLotus corniculatusPlant Sci, 70
- Publisher
- Springer Journals
- Copyright
- Copyright © 1997 by Springer-Verlag
- Subject
- Life Sciences; Biotechnology; Cell Biology
- ISSN
- 0721-7714
- eISSN
- 1432-203X
- DOI
- 10.1007/BF01275497
- Publisher site
- See Article on Publisher Site
Abstract
A procedure for the production of fertile transgenic brassicas via Ri-mediated transformation is reported in this paper. Transgenic hairy root lines were selected for 12 vegetable brassica cultivars and lines representing six varieties: broccoli, Brussels sprouts, cabbage, cauliflower, rapid-cycling (all Brassica oleracea ) and Chinese cabbage ( B. campestris ). Leaf explants or petioles of intact cotyledons were co-cultivated with Agrobacterium strain A4T harbouring various binary vectors. The T-DNA region of all binary vectors contained a neomycin phosphotransferase II gene for kanamycin resistance, in addition to other genes. Hairy root lines grew prolifically on hormone-free medium containing kanamycin. Transgenic shoots were regenerated from all cultivars either spontaneously or after transfer of hairy roots to a hormone-containing medium. Southern analysis confirmed that the plants were transgenic. Plants from all brassica types were successfully transferred to greenhouse conditions. Plants were fertile and segregation analysis confirmed transmission of traits to progeny.
Journal
Plant Cell Reports – Springer Journals
Published: Jun 1, 1997