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Automated Reaction-Rate Method for Determination of Serum Creatinine with the CentrifiChem

Automated Reaction-Rate Method for Determination of Serum Creatinine with the CentrifiChem Abstract An automated kinetic method to determine creatinine in serum with the Jaffé reaction is described. The method does not require deproteinization or adsorption of creatinine on Fuller's earth or ion-exchange resin. A maximum of 29 serum samples, standards, and control sera can be assayed in less than 1.5 min. Interferences by most of the known Jaffé-positive serum constituents were studied and minimized by optimal selection of the time interval during which the reaction rate was measured. Results of precision studies and correlation with a manual method requiring adsorption of creatinine on ion-exchange resin are reported. Spectroscopic data suggest that the product of reaction between picrate and creatinine is a 1:1 adduct of the two starting molecules. centrifugal, batch-parallel analyzer, GeMSAEC, interferences with Jaffé reaction, NMR spectrum of Jaffé reaction product This content is only available as a PDF. © 1971 The American Association of Clinical Chemists, Inc. This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model) http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Clinical Chemistry Oxford University Press

Automated Reaction-Rate Method for Determination of Serum Creatinine with the CentrifiChem

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References (11)

Publisher
Oxford University Press
Copyright
© 1971 The American Association of Clinical Chemists, Inc.
ISSN
0009-9147
eISSN
1530-8561
DOI
10.1093/clinchem/17.8.696
Publisher site
See Article on Publisher Site

Abstract

Abstract An automated kinetic method to determine creatinine in serum with the Jaffé reaction is described. The method does not require deproteinization or adsorption of creatinine on Fuller's earth or ion-exchange resin. A maximum of 29 serum samples, standards, and control sera can be assayed in less than 1.5 min. Interferences by most of the known Jaffé-positive serum constituents were studied and minimized by optimal selection of the time interval during which the reaction rate was measured. Results of precision studies and correlation with a manual method requiring adsorption of creatinine on ion-exchange resin are reported. Spectroscopic data suggest that the product of reaction between picrate and creatinine is a 1:1 adduct of the two starting molecules. centrifugal, batch-parallel analyzer, GeMSAEC, interferences with Jaffé reaction, NMR spectrum of Jaffé reaction product This content is only available as a PDF. © 1971 The American Association of Clinical Chemists, Inc. This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)

Journal

Clinical Chemistry – Oxford University Press

Published: Aug 1, 1971

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