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An identification of the HLA-F null allele in Japanese

An identification of the HLA-F null allele in Japanese Immunogenetics (1997) 45: 466 – 467 Springer-Verlag 1997 SEQUE N CE RE GISTE R Chizuko Uchigiri ? Shinichi Mizuno ? Kaoru Wada Machiko Tsutsumi ? Tohru Kato ? Tadashi Kamiya Kazuo Ozawa Received: 12 November 1996 / Revise: 17 December 1996 The class I major histocompatibility complex (MHC) genes consist of class Ia and class Ib. Class Ia genes (HLA-A,-B, and -C) encode highly polymorphic polypeptides which present antigens to cytotoxic T lymphocytes. In contrast, class Ib genes (HLA-E,-F, and -G) encode significantly fewer polymorphic polypeptides. In the present study, we identified an HLA-F allele which had a non-sense mutation in the exon 3. Single-strand conformational polymorphism (SSCP) Fig. 1mSSCP analysis of HLA-F exon 3 region. PCR products were analysis was carried out for exon 3 of the HLA-F gene. amplified and run in 10% polyacryamide gel with 5% glycerol at The polymerase chain reaction (PCR) primers were 5’- 35 C. The gels were silver stained. Lane 1: Genomic DNA with a AGGGGGGCGGGGCTAGCTGGGCGGGG-3’ (nucleo- normal HLA-F allele. Lane 2: Genomic DNA with an HLA-F null tide 1512 to 1536) for the forward primer and 5’-ACAG- allele. Lane 3: Plasmid DNA containing a normal HLA-F exon 3. Lane http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Immunogenetics Springer Journals

An identification of the HLA-F null allele in Japanese

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References (3)

Publisher
Springer Journals
Copyright
Copyright © 1997 by Springer-Verlag Berlin Heidelberg
Subject
Biomedicine; Immunology; Human Genetics; Gene Function; Cell Biology; Allergology
ISSN
0093-7711
eISSN
1432-1211
DOI
10.1007/s002510050236
Publisher site
See Article on Publisher Site

Abstract

Immunogenetics (1997) 45: 466 – 467 Springer-Verlag 1997 SEQUE N CE RE GISTE R Chizuko Uchigiri ? Shinichi Mizuno ? Kaoru Wada Machiko Tsutsumi ? Tohru Kato ? Tadashi Kamiya Kazuo Ozawa Received: 12 November 1996 / Revise: 17 December 1996 The class I major histocompatibility complex (MHC) genes consist of class Ia and class Ib. Class Ia genes (HLA-A,-B, and -C) encode highly polymorphic polypeptides which present antigens to cytotoxic T lymphocytes. In contrast, class Ib genes (HLA-E,-F, and -G) encode significantly fewer polymorphic polypeptides. In the present study, we identified an HLA-F allele which had a non-sense mutation in the exon 3. Single-strand conformational polymorphism (SSCP) Fig. 1mSSCP analysis of HLA-F exon 3 region. PCR products were analysis was carried out for exon 3 of the HLA-F gene. amplified and run in 10% polyacryamide gel with 5% glycerol at The polymerase chain reaction (PCR) primers were 5’- 35 C. The gels were silver stained. Lane 1: Genomic DNA with a AGGGGGGCGGGGCTAGCTGGGCGGGG-3’ (nucleo- normal HLA-F allele. Lane 2: Genomic DNA with an HLA-F null tide 1512 to 1536) for the forward primer and 5’-ACAG- allele. Lane 3: Plasmid DNA containing a normal HLA-F exon 3. Lane

Journal

Immunogenetics – Springer Journals

Published: Apr 9, 1997

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