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Abstract
Incubation of a 1 : 1 mixture of δ-(-α-aminoadipoyl)--cysteinyl--valine (,,-ACV) and ,,-A[3,3-H]CV with a cell-free extract of isopenicillin N synthetase from results in preferential conversion of the fully protiated substrate into isopenicillin N; alternatively a similar experiment with a 1 : 1 mixture of ,,-ACV and ,,-AC[3-H]V gave isopenicillin N without isotopic discrimination between the two substrates.